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[摘要]
近红外光谱技术是一种快速、无损的分析方法,化学计量学可以用于复杂数据的分析处理。将近红外光谱技术与化学计量学结合,可以作为中药制药过程控制的一种强有力的工具。近年来,近红外光谱技术已经较为广泛地应用于中药制药过程控制或在线监测。将重点介绍化学计量学和近红外光谱技术在中成药液体制剂方面的应用进展,主要应用领域包括:定性、定量分析方法和中成药液体制剂生产过程监控。
[Key word]
[Abstract]
Objective To establish a method for simultaneous determination of the contents of six main active constituents (neochlorogenic acid, chlorogenic acid, rutin, hyperin, isoquercitrin, and quercitrin) in Houttuyniae Herba by quantitative analysis of multi-components by single marker (QAMS). Methods The chromatographic separation was achieved on a Kromasil C18 column (250 mm × 4.6 mm, 5 μm), the mobile phase was acetonitrile-0.1% phosphoric acid aqueous with gradient elution and changeable wavelength detection. Chlorogenic acid was used as the reference, the calibration factor of other five constituents to that of chlorogenic acid was calculated respectively to get each relative calibration factor. And the contents of the active constituents were calculated by the relative calibration factor to realize QAMS. At the same time, the external standard method was used to detect the contents of the six constituents, comparing the two methods to validate the accuracy and feasibility of the new method. Results No significant differences were found in the quantitative results of the six active constituents in Houttuyniae Herba determined by measured value and calculated value. The amounts of the six active constituents in the different parts of Houttuyniae Herba were found to be varied, chlorogenic acids and flavonoids mainly distributed in the leaves. Conclusion The active constituents of Houttuyniae Herba mainly distribute in the leaves. The QAMS is feasible and accurate to evaluate the contents of the six active constituents in Houttuyniae Herba.
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[基金项目]
江苏省科技支撑计划工业项目(BE2012011);南京市科技公共服务平台建设项目(201105007)