目的 建立鱼腥草不同部位中新绿原酸、绿原酸、芦丁、金丝桃苷、异槲皮苷及槲皮苷的一测多评测定方法。方法 以Kromasil C¹⁸色谱柱（250 mm×4.6 mm，5 μm），乙腈-0.1%磷酸溶液梯度洗脱，分段变波长测定。以绿原酸为参照物建立与其余成分的相对校正因子，并计算其量，实现一测多评。同时采用外标法测定该6个成分的量，并比较二者的差异，以验证一测多评法的准确性和可行性。结果 鱼腥草中6个成分的计算值与实测值间无显著差异，鱼腥草不同部位6个成分量有所不同，绿原酸类和黄酮类主要分布在叶中。结论 鱼腥草的药效成分主要集中在叶中，本实验建立的一测多评法可行准确，可以用来对鱼腥草进行质量控制。

Objective To study the genetic diversity of Dipsacus asper from different populations and provide a reference for the rational utilization of its germplasm. Methods The genetic diversity of the 14 populations of D. asper was analyzed by SRAP molecular markers. Results Ten pairs of primers produced 124 sites, among which 102 were polymorphic sites. The percentage of polymorphic loci (PPL) was 82.26%. The Nei’s genetic diversity index (H) and the Shannon’s information index (I) were 0.280 0 and 0.435 3, respectively. At the population level, PPL was 53.92%, H was 0.121 2－0.244 0, and I was 0.179 6－0.361 1. The genetic diversity values of the five populations were relatively high, and the populations had the characteristics of high altitude and microhabitat. Genetic differentiation coefficient (Gst) was 0.293 0, gene flow (Nm) was 1.206 4. Cluster analysis based on genetic similarity indicated that the 14 populations could be divided into three groups. Conclusion The genetic diversity among the populations of D. asper was at relatively high level. The genetic variance of D. asper mainly existed within the populations. The high genetic diversity could be attributed to the geographical position (altitude) and climate, while geographic isolation (microhabitat) was another important factor for the genetic variance within the populations.

泸州市2010年重点科技项目（泸市财企 [2010] 41号）；泸州医学院2010年青年基金（泸医院 [2010] 108号）