目的 筛选油橄榄叶抗糖尿病活性部位。方法 应用回流提取法和大孔吸附树脂技术，从油橄榄叶中提取6个部位（A～F）。对6个部位进行α-淀粉酶抑制实验、蛋白质非酶糖基化抑制实验和四氧嘧啶致小鼠糖尿病模型实验，综合评价和筛选抗糖尿病活性部位。结果 C～F部位对α-淀粉酶、蛋白质非酶糖基化均显示明显的抑制活性，其中以D部位活性最强，且呈量效关系。C、D部位降血糖作用较明显，且以D部位活性最显著。结论 油橄榄叶具有潜在抗糖尿病活性，其中D部位的活性尤为显著。

Objective To screen the anti-diabetic effective fraction in the leaves of Olea europaea. Methods Different fractions (Frs. A—F) were prepared using reflux extraction and separation with macroporous resins. α-Amylase system, non-enzymatic glycation system, and alloxan-induced diabetic models of mice were used to evaluate the anti-diabetic activities of the different fractions, respectively. Results Frs. C—F fractions showed the significant inhibitory activities against α-amylase system and non-enzymatic glycation system. Especially, fraction D showed the best anti-diabetic activity compared with the other fractions, which also displayed a good dose-effect relationship. The anti-diabetic activities of Frs. C and D were both obvious and Fr. D showed more significance. Conclusion The leaves of O. europaea show the potential anti-diabetic activity and the most effective fraction is ascribed to Fr. D.

中国科学院“百人计划”资助项目（2007-0629141051）；国家自然科学基金资助项目（20974116）