目的 研究黄连水提取物中多组分的不同组合与吸收的相关性，探索甄别中药多组分吸收相互影响的方法。方法 采用Caco-2细胞单层模型，以黄连水提取物中的多组分及其不同组合为受试药物，通过HPLC法对各组分同时进行定量，以各组分单独转运时的固有表观渗透系数作为参照，采用最小二乘法多元线性回归进行拟合，获得各组分间表观渗透系数的作用强度与各组分浓度及其不同组合间的相关性，分析多组分在吸收中的相互作用类型及强度。结果 甄别出黄连碱、药根碱、小檗碱和巴马汀跨Caco-2细胞单层膜吸收的相互作用，得到影响各组分吸收的单组分因素和多组分组合因素，获得影响其吸收的定量关系。结论 本实验采用数学方法研究黄连多组分在吸收中的相互作用切实可行。

Objective To research the correlation between compositions and absorption of the multi-components in Coptidis Rhizoma and to explore the methods suitable for identifying the interactions of multi-components in Coptidis Rhizoma during the absorption process. Methods Samples of various compositions by their different combinations of each component in Coptidis Rhizoma were designed and prepared using Caco-2 monolayer model. Using HPLC method for the simultaneous quantitative determination, taking the inherent apparent permeability coefficients (P_app) by the individual transport of each component as reference, the least square multi-linear regression was used for data fitting to obtain the correlation between interaction intensity of P_app and concentration of each component by their different combinations and to analyze the interaction type and intensity of multi-components during the absorption. Results The quantitative relationship of multi-component interactions for coptisine, jatrorrhizine, berberine, and palmatine was obtained, respectively. The main contributors for the multi-component interaction, including single component and interactive products of the different components, were also identified. The quantitative relationship affecting the absorption was also obtained. Conclusion A well-designed method for studying the multi-component interactions of Coptidis Rhizoma using mathematical method is practicable.

国家科技重大专项资助项目（2012ZX09301001-001）