[关键词]
[摘要]
目的 优选虎耳草Saxifraga stolonifera抗前列腺癌活性部位的提取工艺。方法 以岩白菜素、槲皮素-5-O-β-D-吡喃葡萄糖苷、槲皮素-3-O-L-鼠李糖苷、槲皮素以及总黄酮的提取率为考察指标,采用单因素试验结合Box-Behnken中心组合设计方法进行提取工艺优化研究。结果 虎耳草抗前列腺癌活性部位的最佳提取工艺条件为回流提取,乙醇体积分数60.04%,料液比例1∶13.79,提取时间89.98 min,提取温度80 ℃,在此条件下岩白菜素、槲皮素-5-O-β-D-吡喃葡萄糖苷、槲皮素-3-O-L-鼠李糖苷和槲皮素以及总黄酮的提取率分别为97.15%、98.13%、98.37%、97.82%、96.33%。结论 采用响应面法优化了虎耳草中抗前列腺癌活性部位的提取工艺,对虎耳草的综合利用以及产业化发展提供了科学依据。
[Key word]
[Abstract]
Objective To optimize the extracting technology for active fractions from Saxifraga stolonifera with anti-prostate cancer activity. Methods Using the extraction rates of bergenin, quercetin-5-O-β-D-glucopyranoside, quercetin-3-O-L-rhamnopyranoside, quercetin, and flavonoids as indexes, the single factor experiment combined with Box-Behnken design was used to optimize the extracting technology. Results The optimal extraction conditions were as follows: ethanol volume fraction 60.04%, liquid-solid ratio of 1∶13.79 for 89.98 min, and at the temperature of 80 ℃. Under these conditions, the extraction rates of bergenin, quercetin- 5-O-β-D-glucopyranoside, quercetin-3-O-L-rhamnopyranoside, quercetin, and flavonoids were 97.15%, 98.13%, 98.37%, 97.82%, and 96.33%, respectively. Conclusion The response surface analysis could optimize the extracting technology of active fraction from S. stolonifera with the anti-prostate cancer activity, which provides the basis for the comprehensive utilization and industrialization of S. stolonifera.
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[基金项目]
贵州省中药现代化科技产业研究开发专项项目(黔科合中药字 [2010] 5023号);贵阳市科学技术计划项目([2010] 筑科合同字第1-中-23号);贵州省科技创新人才团队建设项目(黔科合人才团队[2011]4008);贵州省教育厅特色重点实验室项目(黔科合KY[2012]005号)