[关键词]
[摘要]
目的 明确板蓝根Isatidis Radix抑菌活性的物质基础。方法 采用管碟法,以抑菌系数作为评价指标,检测板蓝根总提取物、总有机酸部位、总生物碱部位、总核苷部位、总蒽醌部位对大肠杆菌AG100A的抑制作用,确定抑菌活性最强部位后,建立10批不同产地板蓝根样品的HPLC指纹图谱,应用主成分分析法将HPLC指纹图谱共有峰的峰面积与抑菌系数进行相关分析。结果 板蓝根总有机酸部位对大肠杆菌AG100A的抑菌作用最强,对其建立的HPLC指纹图谱有共有峰21个,与抑菌作用关系密切的6个共有峰分别为2、10、16、18、20、21号,其中10、18号分别为水杨酸和苯甲酸。结论 板蓝根总有机酸部位有较强的抑菌活性,其中苯甲酸、水杨酸等化学成分与抑菌活性关系密切。
[Key word]
[Abstract]
Objective To study the active components with anti-bacterial effect in Isatidis Radix. Methods Using cylinder-plate method with bacteriostatic coefficient as the index, the anti-Escherichia coli AG100A effects of five extracts (total extract, tatal organic acid extract, total alkaloids extract, tatal nudeoside extract, and total anthraquinone extract) from Isatidis Radix were studied. After the determination of the most active extract, HPLC fingerprints on 10 batches of Isatidis Radix from different habitats were established. The correlations between areas of common peaks and bacteriostatic coefficients were analyzed by principal component analysis (PCA). Results The total organic acid extract showed the best anti-bacterial effect, with 21 common peaks in HPLC fingerprint. The results of the PCA demonstrated that the common peaks 2, 10, 16, 18, 20, and 21 were connected closely with the antibacterial activity, among which the common peaks 10 and 18 were proved to be salicylic acid and benzoic acid, respectively. Conclusion The total organic acid extract from Isatidis Radix shows the significant antibacterial effect, in which salicylic acid and benzoic acid are the active chemical constituents with the antibacterial activity.
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[基金项目]
国家自然科学基金资助项目(30873381)