[关键词]
[摘要]
目的 制备新藤黄酸纳米结构脂质载体并表征其药剂学性质。方法 采用乳化蒸发-低温固化法制备新藤黄酸纳米脂质载体(GNA-NLC),正交试验设计优化最佳工艺处方,并对其包封率、平均粒径及Zeta电位等性质进行考察。结果 优化后处方制备的GNA-NLC多为圆整、实体的类球形,平均粒径为(144.07±1.44)nm,多分散系数为0.24±0.01,Zeta电位为(?28.03±0.29)mV,包封率为(84.65±0.98)%,载药量为(4.21±0.05)%;DSC显示GNA纳米粒确已形成,并且GNA以非晶态分布在基质中。结论 乳化蒸发-低温固化法能成功制备GNA-NLC,工艺简单,易于控制。
[Key word]
[Abstract]
Objective To prepare and characterize gambogenic acid loaded nanostuctured lipid carrier (GNA-NLC). Methods GNA-NLC was prepared by emulsion evaporation-low temperature solidification method, and its optimal prescription was selected by orthogonal test design. The characteristics, such as encapsulation efficiency (EE), average diameter, and Zeta potential, were studied. Results GNA-NLC had a spherical shape with smooth surface with the average diameter of (144.07 ± 1.44) nm, polydispersity index (PI) of 0.24 ± 0.01, Zeta potential of (?28.03 ± 0.29) mV. The EE and drug loading were (84.65 ± 0.98)% and (4.21 ± 0.05)%, respectively. DSC indicated that GNA was dispersed as non-crystalline in matrix. Conclusion Emulsion evaporation-low temperature solidification method is easy, simple, and feasible to prepare GNA-NLC.
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[基金项目]
国家“重大新药创制”科技重大专项(2009ZX09103-399);安徽省高校省级自然科学研究重点项目(KJ2011A190)