目的 研究姜黄中姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素的光稳定性，并对双去甲氧基姜黄素光化学反应产物进行考察。方法 姜黄的甲醇提取液于棕色量瓶储存，在自然光/避光条件下放置0、1、2、4、6、8 h后，HPLC法测定其指标成分姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素量的变化；LC-MS法分析双去甲氧基姜黄素光化学反应产物。结果 姜黄素、去甲氧基姜黄素在自然光/避光条件下均有良好的稳定性；双去甲氧基姜黄素在避光条件下稳定，见光条件下发生光化学反应。结论 姜黄素和去甲氧基姜黄素具有良好稳定性，双去甲氧基姜黄素在自然光照射下不稳定，因此，姜黄药材分析供试液应于棕色量瓶中避光保存。

Objective To study the photostability of curcumin (Cur), demethoxycurcumin (DMCur), and bisdemethoxycurcumin (BDMCur) in the rhizomes of Curcuma longa, and to investigate the photochemical conversion product of BDMCur. Methods The stock solution of the extracts from the rhizomes of C. longa was kept in brown volumetric flasks. Then the absorbances of Cur, DMCur, and BDMCur were determined by HPLC analysis, and the solutions were placed in the daylight or daylight/dark conditions for 0, 1, 2, 4, 6, and 8 h. The photochemical conversion products of BCMCur were detected by LC-MS analysis. Results Both Cur and DMCur were stable in the daylight and daylight/dark conditions. BDMCur was liable to photochemical reaction in the daylight condition. Conclusion Both Cur and DMCur have good photostabilities, but BDMCur is not stable in daylight condition. As a result, the sample solution of the rhizomes of C. longa should be conserved in dark.

国家科技重大专项“辽宁省国家创新药物孵化（本溪）基地建设”课题（2010ZX09401-304-504）