目的 研究提取文殊兰中生物碱的最佳工艺条件。方法 在单因素的基础上运用Box-Behnken中心组合设计以提取温度，提取时间，乙醇的体积分数3个因素3水平的实验模型，建立提取回归方程。以响应曲面法（RSM）对实验结果进行分析，对文殊兰中生物碱的提取工艺进行优化。结果 模拟得到的最优条件为提取时间4.13 h，乙醇体积分数为66.36%，提取温度为71.64 ℃，此条件下生物碱的最高提取率为3.422 mg/g。为了方便实际操作，修正条件为提取时间4.1 h，乙醇体积分数为66%，提取温度72 ℃。验证实验结果平均提取率为3.563 mg/g，与模型预测值较为接近。结论 曲面响应法可以对文殊兰中生物碱的提取工艺进行优化。

Objective To optimize the extracting technology for alkaloids from the seeds of Crinum asiaticum. Methods On the basis of the single factor test, the effects of extracting time, volume of ethanol, and extracting temperature were studied by the orthogonal test developed by Box-Behnken central composite test design and the relative equation was established. Using the response surface method (RSM), the result was analyzed and the extracting process was optimized. Results The optimized conditions were as follows: the extracting time was 4.13 h, the volume of ethanol was 66.36%, the extrating temperature was 71.64 ℃, and the yield was 3.422 mg/g. In order to facilitate the practice, the conditions were modified as follows: the extracting time was 4.1 h, the volume of ethanol was 66%, and the extracting temperature was 72 ℃. The experimental results was verified with an average yield of 3.563 mg/g, which is close to the model predicted value. Conclusion The RSM technology optimization for extracting alkaloids from the seeds of C. aticum is suitable.

国家自然科学基金项目（81274067）；黑龙江省自然科学基金资助项目（D201126）；黑龙江省教育厅科技项目（1211135）；教育部科学技术研究重点项目（211045）；教育部博士点基金项目（20112332110003）；黑龙江省教育厅科技重点项目（12511z011）；黑龙江省研究生创新基金项目（YJSCX2011-165HLJ）