目的 研究高压脉冲电场法对黄花乌头中关附甲素的最佳提取工艺，为工业化生产提供可靠的实验数据和理论依据。方法 采用正交试验设计优化高压脉冲电场法快速提取方法，HPLC法测定关附甲素的量。结果 提取黄花乌头关附甲素的最佳提取工艺条件为以90%乙醇为提取溶剂，电场强度20 kV/cm，脉冲数为10，料液比1∶14，影响因素由大到小依次为电场强度、料液比、脉冲数；此法提取黄花乌头中的关附甲素提取率达到0.391%，浸膏得率为39.92%。结论 本提取工艺可行、经济、省时、回收率高。

Objective To optimize the extracting technology for guanfubase A from Aconitum coreanum by high-voltage pulsed electric field method and to provide the reliable experimental data and theoretical basis for the industrial production. Methods The content of guanfubase A was detected by HPLC in orthogonal test. Results The optimal formulation was as follows: high voltage of 20 kV/cm, impulsion frequency of 10, and solid-to-solvent ratio of 1∶14, with 90% ethanol as extracting solvent, and the influence factor in size was voltage, impulsion frequency, and solid-to-solvent ratio. The guanfubase A content and extract yield were up to 0.391% and 39.92% in A. coreanum, respectively. Conclusion The extracting technology is feasible and economic, which has the advantage of time-saving and high recovery rate.

吉林省自然科学基金资助项目（201115131）；吉林市科技局项目（201222306）