目的 比较超高效液相色谱（UPLC）法与HPLC法分析化橘红总黄酮指纹图谱的效果。方法 分别采用UPLC法与HPLC法对17批化橘红药材的黄酮类成分进行指纹图谱分析。结果 分别建立了化橘红黄酮类成分的UPLC与HPLC指纹图谱共有模式。其中UPLC法标识出21个共有峰，HPLC法标识出17个共有峰，17批样品的相似度均大于0.9。结论 2种方法均可用于化橘红的质量控制，UPLC法较HPLC法更高效、快速、灵敏。

Objective To compare the UPLC and HPLC fingerprints of flavonoids in Citri Grandis Exocarpium (CGE). Methods The HPLC and UPLC methods for analyzing the fingerprint of 17 samples were established respectively. Results There were 21 mutual peaks selected from UPLC chromatography and 17 mutual peaks selected from HPLC chromatography. The similarity of 17 samples was over 0.9. Conclusion Both the UPLC and HPLC methods could be used for the quality control of CGE. Compared to HPLC, the UPLC method is efficient, quickly, and sensitive.

国家科技部“十二五”科技支撑计划项目（2011BAI01B02）；广东省科技计划项目（2012A030100005）