目的 通过观察Z-藁本内酯对肿瘤坏死因子-α（TNF-α）诱导的人脐静脉内皮细胞（HUVEC）细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）及核因子-κB（NF-κB）表达的影响，探讨其对炎性因子损伤内皮细胞的保护作用及其机制。方法 向体外培养的HUVEC中加入TNF-α 10 ng/mL造成细胞损伤，同时加入不同浓度（5、10、20 μmol/L）的Z-藁本内酯，共同孵育24 h，MTT法检测各组细胞活力，ELISA法测定细胞培养液中ICAM-1和VCAM-1的量，Western blotting法检测各组细胞NF-κB蛋白表达。结果 与对照组比较，TNF-α可造成HUVEC明显损伤（P＜0.05、0.01），显著增加HUVEC中ICAM-1和VCAM-1的表达（P＜0.01）。与模型组比较，Z-藁本内酯可以剂量相关地降低ICAM-1（P＜0.01）和VCAM-1（P＜0.05）的表达，显著减少细胞NF-κB蛋白的表达（P＜0.05）。结论 Z-藁本内酯可减轻TNF-α对HUVEC的损伤，其作用机制可能与通过抑制NF-κB的激活，进而减少ICAM-1和VCAM-1的表达有关。

Objective To observe the effect of Z-ligustilide on the expression of tumor necrosis factor-α (TNF-α)-induced human umbilical vein endothelial cells (HUVEC) intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and nuclear factor kappa B (NF-κB), and to investigate the protection on endothelial cells injured by inflammatory cytokines and its mechanism. Methods TNF-α (10 ng/mL) was added to in vitro cultured HUVEC to induce the cell injury. After co-incubated with the different concentration (5, 10, and 20 μmol/L) of Z-ligustilide for 24 h, the cell viability in each group was measured by MTT. The levels of ICAM-1 and VCAM-1 were assayed using enzyme linked immunosorbent assay (ELISA). Western blotting was used to detect the expression of NF-κB protein of cell in each group. Results TNF-α could induce the obvious injury of HUVEC compared with the control group (P < 0.05, 0.01), and increase the expression of ICAM-1 and VCAM-1 in HUVEC (P < 0.01). Compared with the model group, Z-ligustilide could dose-dependently reduce the expression of ICAM-1 (P < 0.01) and VCAM-1 (P < 0.05) and obviously decrease the NF-κB expression (P < 0.05). Conclusion Z-ligustilide could attenuate TNF-α-induced injury in HUVEC via suppressing the activation of NF-κB and inhibiting the excessive expression of ICAM-1 and VCAM-1.

科技部国际合作课题（2009DFB30510）