目的 研究薤白Allii Macrostemonis Bulbus的皂苷类化学成分。方法 采用正相、反相硅胶柱色谱进行分离纯化，根据理化性质和波谱数据分析鉴定化合物的结构。结果 从薤白70%乙醇提取物中分离鉴定了3个皂苷类化合物，分别为5β-螺甾-25(27)-烯-3β, 12β-二醇-3-O-β-D-吡喃葡萄糖基-(1→2)-β-D-吡喃半乳糖苷（1）、(25R)-5β-螺甾-3β, 12β-二醇-3-O-β-D-吡喃葡萄糖基-(1→2)-β-D-吡喃半乳糖苷（2）、5β-螺甾-25(27)-烯-2β, 3β-二醇-3-O-β-D-吡喃葡萄糖基-(1→2)-β-D-吡喃半乳糖苷（3）。结论 化合物1为1个新化合物，命名为薤白皂苷S，化合物2为首次从该植物中分离得到。

Objective To study the steroidal saponins from Allii Macrostemonis Bulbus. Methods The silica gel and ODS column chromatographies were used for the isolation and purification of compounds from Allii Macrostemonis Bulbus. Their structures were identified through comparing with references and spectral analyses. Results Three steroidal saponins were isolated from the 70% ethanol extract from Allii Macrostemonis Bulbus and identified as 5β-spirostane-25(27)-en-3β,12β-diol-3-O-β-D-glucopyranosyl- (1→2)-β-D-galactopyranoside (1), (25R)-5β-spirostane-3β,12β-diol-3-O-β-D-glucopyranosyl-(1→2)-β-D-galactopyranoside (2), and 5β-spirostane-25(27)-en-2β, 3β-diol-3-O-β-D-glucopyranosyl-(1→2)-β-D-galactopyranoside (3). Conclusion Compound 1 is a new compound named macrostemonoside S. Compound 2 is isolated from Allii Macrostemonis Bulbus for the first time.

“重大新药创制”国家科技重大专项（2011ZX09307-002-01）