[关键词]
[摘要]
目的 对冬虫夏草真菌蝙蝠蛾拟青霉菌Paecilomyces hepiali发酵菌丝体进行化学成分研究。方法 利用硅胶柱、凝胶柱、高效液相色谱等技术对蝙蝠蛾拟青霉菌发酵菌粉的95%乙醇提取物进行分离纯化,并通过核磁共振谱、质谱等谱学方法鉴定化合物结构。结果 从95%乙醇提取物的醋酸乙酯萃取部分分离得到11个化合物,分别鉴定为 (22E, 24R)-麦角甾-7, 9, 22-三烯-3β-醇(1)、(22E, 24R)-麦角甾-7, 22-二烯-3β, 5α, 6β-三醇(2)、(22E, 24R)-麦角甾-6β-甲氧基-7, 22-二烯-3β, 5α-二醇(3)、胡萝卜苷(4)、环 (亮-色) 二肽(5)、环 (异亮-色) 二肽(6)、环 (缬-色) 二肽(7)、4-(2-甲酰基-5-羟甲基-1-氢-吡咯-1-基) 丁酸(8)、4-(2-甲酰基-5-甲氧基甲基-1-氢-吡咯-1-基) 丁酸(9)、5-羟甲基糠酸(10)、琥珀酸(11)。结论 化合物1、2、4~9均为首次从拟青霉(瓶梗青霉)属真菌中分离得到。
[Key word]
[Abstract]
Objective To investigate the chemical constituents in the fermented mycelia from Paecilomyces hepiali. Methods The chemical constituents were isolated and purified by repeated silica gel column, Sephadex LH-20, and semi-preparative HPLC chromatography, and their structures were identified by spectroscopic analyses (MS and NMR). Results Eleven known compounds were isolated from the ethyl acetate fraction of 95% ethanol extract and were identified as ergosterol D (1), cerevisterol (2), 6-O- methylcerevisterol (3), daucosterol (4), cyclo (L-Leu-L-Trp) (5), cyclo (L-Ile-L-Trp) (6), cyclo (L-Val-L-Trp) (7), 4-[formyl-5- (hydroxymethyl)-1H-pyrrol-1-yl] butanoic acid (8), 4-[formyl-5-(methoxymethyl)-1H-pyrrol-1-yl] butanoic acid (9), 5-hydroxyl- methylfuroic acid (10), and succinic acid (11). Conclusion Compounds 1, 2, and 4—9 are isolated from the fungi of Paecilomyces Bain for the first time.
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[基金项目]
国家自然科学基金资助项目(90713040,81273401,81202420);上海市科委科研项目(1DZ1921203)