目的 建立牡丹皮的HPLC指纹图谱。方法 色谱柱为Dikma Diamonsil C₁₈色谱柱（250 mm×4.6 mm，5 μm）；以乙腈-水（含0.085%磷酸）为流动相梯度洗脱；柱温35 ℃；体积流量1 mL/min；检测波长254 nm（0～21.5 min），230 nm（21.5～45 min），254 nm（45～60 min）；进样量20 μL。采用高分辨LC-MS/MS技术进行色谱峰指认。结果 该方法精密度、稳定性和重复性良好。采用高分辨LC-MS/MS方法对20个共有峰中的18个色谱峰进行了定性鉴别。采用该方法测定了10批不同产地的牡丹皮，其相似度均在0.96以上。结论 本实验为牡丹皮的全面质量评价奠定了基础。

Objective To establish the fingerprint of Moutan Cortex by HPLC. Methods The chromatographic fingerprint was obtained with Dikma Diamonsil C₁₈ column (250 mm × 4.6 mm, 5 μm) and gradient eluted with acetonitrile and water (with 0.085% phosphoric acid). The column temperature was maintained at 35 ℃, and the flow rate was 1 mL/min. The detection wavelength was 254 (0—21.5 min), 230 (21.5—45 min), and 254 nm (45—60 min). The injection volume was 20 μL. The peaks were identified by LC-MS/MS. Results The method had good precision, stability, and repeatability. Eighteen chromatographic peaks among 20 common peaks were identified by LC-MS/MS. Ten batches of Moutan Cortex were determined, and the similarities were above 0.96. Conclusion The study lays the foundation for the full quality evaluation of Moutan Cortex.

国家“重大新药创制” 科技重大专项课题（2011ZX09201-201-13，2012ZX09301003-001-004）