目的 研究制川乌与法半夏不同配伍组合中乌头类生物碱的变化趋势，阐明两药配伍后的化学物质基础。方法 采用LC-MS技术分析久煎处理的不同配伍组合，结合药液pH值的变化，运用化学计量学方法寻找、分析差异标记物。结果 制川乌与法半夏不同配伍组合化学成分的量差异明显，随着法半夏比例的增大，药液pH值呈上升趋势，乌头类双酯型生物碱的量整体呈下降趋势。结论 制川乌与法半夏配伍后毒性物质减少，这可能与法半夏中的某些成分影响了乌头类生物碱的稳定状态有关。

Objective To investigate the change tendency of Aconitum alkaloids in Aconiti Cocta Radix (ACR) combined with Pinelliae Praeparatum Rhizoma (PPR) in different ratios, and to elaborate the chemical material basis of the compatibility of ACR and PPR. Methods LC-MS technology was used to analyze the decoctions with different ratios after long-time frying, and chemometrics was applied to identify and analyze the chemical markers according to the change of pH value. Results There were the significant differences in chemical constituents of ACR-PPR compatibility with different ratios. The pH value increases when the ratios of PPR increasing. However, the content of diester diterpene alkaloids was totally decreasing. Conclusion The toxic substance is decreased when ACR and PPR combined, which could be related to some constituents in PPR affecting the stability of Aconitum alkaloids.

国家重点基础研究发展计划（“973”计划）（2011CB505300，2011CB505302）