[关键词]
[摘要]
目的 研究常用中药附子的化学成分。方法 利用硅胶柱色谱、中性氧化铝柱色谱、Sephadex LH-20凝胶柱色谱、中压MCI柱色谱等方法分离纯化,通过核磁共振谱、质谱等波谱数据鉴定化合物结构。结果 从附子中分离得到18个化合物,分别鉴定为新乌头碱(1)、乌头碱(2)、次乌头碱(3)、苯甲酰次乌头碱(4)、苯甲酰新乌头碱(5)、苯甲酰乌头碱(6)、展花乌头宁(7)、塔拉乌头胺(8)、尼奥灵(9)、多根乌头碱(10)、附子灵(11)、准格尔乌头碱(12)、异塔拉乌头定(13)、川附子碱B(14)、16β-羟基心瓣翠雀碱(15)、哥伦乌头碱(16)、苯甲酸(17)、6-hydroxymethyl-3-pyridinol(18)。结论 化合物18为首次从该属植物中分离得到,化合物15~17为首次从该植物中分离得到。
[Key word]
[Abstract]
Objective To investigate the chemical constituents of Aconiti Lateralis Radix Praeparata. Methods The constituents were isolated by silica gel, neutral alumina, Sephadex LH-20 gel, and medium-pressure MCI columns, and their structures were identified by chemical properties and spectroscopic analyses. Results Eighteen compounds were isolated and their structures were elucidated as mesaconitine (1), aconitine (2), hypaconitine (3), 14-benzoylhypaconitine (4), 14-benzoylmesacontine (5), 14-benzoyaconitine (6), chasmanine (7), talatisamine (8), neoline (9), karakoline (10), fuziline (11), songorine (12), isotalatizidine (13), senbusine B (14), 16β-hydroxycardiopetaline (15), columbianine (16), benzoic acid (17), and 6-hydroxymethyl-3-pyridinol (18). Conclusion Compounds 15—18 are obtained from this plant for the first time.
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[基金项目]
国家“973”计划项目(2009BC522805);国家重大新药创制科技重大专项(2011ZX09307-002-01,2012ZX09301-001-001)