目的 观察苓桂术甘汤对慢性心力衰竭模型大鼠心肌组织肿瘤坏死因子-α（TNF-α）蛋白及mRNA表达、血清核因子-κB（NF-κB）、白细胞介素-1β（IL-1β）水平的影响，探讨苓桂术甘汤防治慢性心衰的作用机制。方法 采用冠状动脉结扎法制备慢性心衰大鼠模型，造模4周后将模型大鼠随机分为模型组，卡托普利（4.375 mg/kg）阳性对照组，苓桂术甘汤低、中、高剂量（生药2.1、4.2、8.4 g/kg）组，另设假手术组，每天给药1次，连续给药4周。Western blotting、RT-PCR法分别检测各组大鼠心肌组织TNF-α蛋白及mRNA的表达，ELISA法检测血清中NF-κB、IL-1β水平。结果 与假手术组相比，模型组大鼠心肌组织TNF-α蛋白及mRNA表达增强，血清NF-κB、IL-1β水平显著升高（P＜0.01）；与模型组相比，苓桂术甘汤及卡托普利均能显著抑制模型大鼠心肌组织TNF-α蛋白及mRNA表达、降低模型大鼠血清NF-κB、IL-1β水平（P＜0.05、0.01）。结论 苓桂术甘汤干预慢性心衰的机制与其调节细胞因子网络有关。

Objective To study the mechanism of Linggui Zhugan Decoction (LZD) on chronic heart failure (CHF), through observing the expression of TNF-α and TNF-α mRNA in myocardial tissue and the levels of NF-κB and IL-1β in serum of model rats with CHF. Methods Model of CHF was produced by ligation of coronary artery. Four weeks after this procedure, rats were randomly classified into model, Captopril (4.375 mg/kg), and low-, mid-, and high-dose (2.1, 4.2, and 8.4 g/kg) of LZD groups. Sham group and the other five groups were ig administered once daily for consecutive 4 weeks. The expression of TNF-α and TNF-α mRNA in myocardial tissue was detected by Western blotting and RT-PCR and the levels of NF-κB and IL-1β in serum were detected by ELISA. Results The expression of TNF-α and TNF-α mRNA of myocardial tissue was enhanced and the levels of NF-κB and IL-1β in serum of model group were significantly increased compared with Sham group (P < 0.01). The low-, mid-, and high-dose of LZD and Captopril groups could inhibit the expression of TNF-α and TNF-α mRNA in myocardial tissue and decrease the levels of NF-κB and IL-1β in serum significantly compared with the model group (P < 0.05, 0.01). Conclusion The mechanism of LZD interfering CHF seems to be related to the regulation of the cytokine network inflammatory factors.

国家自然科学基金面上项目（30973707）；安徽省自然科学基金资助项目（070413262X）；安徽省科技计划项目（10021303024）