目的 对大黄附子汤进行血清药物化学初步研究，通过分析入血成分探讨该复方发挥药效的物质基础。方法 采用血清药物化学的研究方法，同时建立大黄附子汤及大鼠ig给予大黄附子汤后血清HPLC指纹图谱，通过分析比较分别给予大黄附子汤、单味生药所得血清样品，初步鉴定大黄附子汤在大鼠血清中的移行成分。结果 大鼠ig给予大黄附子汤后，血清中发现18个入血成分，其中14个为代谢产物；4个为原型成分。结论 大黄附子汤入血成分主要来自大黄和附子，复方血清药物化学研究为深入阐述大黄附子汤药效物质基础提供依据。

Objective To carry out a preliminary study on serum parmacochemistry of Dahuang Fuzi Decoction (DFD) and to research the pharmacodynamic material basis through analyzing the constituents absorbed into blood. Methods HPLC fingerprints of DFD and serum of rats after DFD treatment were established with serum pharmacochemistry method. The serum samples collected after DFD and single crude drug treatments were compared, the constituents absorbed into the serum after DFD ig administration was determined. Results After the rats were ig administered with DFD, 18 transitional constituents to blood were detected, among which 14 were metabolites and 4 were prototype constituents. Conclusion After DFD treatment, the constituents absorbed into blood are mainly from Rhei Radix et Rhizoma and Aconiti Lateralis Radix Praeparata, and the serum pharmacochemistry signs could provide the evidence for the further research of the material basis of the decoction.

国家自然科学基金面上项目（81073022）