目的 研究不同质量浓度的KNO₃、KMnO₄、H₂O₂、GA₃以及蒸馏水浸种不同时间，对桔梗种子萌发及幼苗生长的影响。方法 采用双层滤纸培养法，在25 ℃恒温，12 h光照的光照培养箱中将浸种后的种子进行培养，观察记录种子的发芽势、发芽率、发芽指数及幼苗的根长和苗高，并对数据进行统计分析。结果 0.150 g/L GA₃浸种24 h是打破桔梗种子休眠、促进桔梗种子萌发和幼苗生长的最佳浸种处理方法；此外，5 mg/mL KNO₃浸种处理12 h也是有效的浸种处理方法。结论 筛选出桔梗种子萌发的最适浸种试剂及浸种时间，对桔梗的种子育苗及人工栽培具有重要指导意义。

Objective To investigate the effects of different soaking treatments by KNO₃, KMnO₄, H₂O₂, GA₃, and distilled water for different times on seed germination and seedling growth of Platycodon grandiflorum. Methods Adopting a double-layer filter paper culture method, the seeds were cultured in the 12 h illumination light incubator at 25 ℃, and the germination energy, germination percentage, germination index of the seeds, and the root length and the shoot height of the seedling were recorded. Then the data were analyzed. Results The best soaking treatment to break seed dormancy, promote the seed germination, and improve the seedling growth of P. grandiflorum was with 0.150 g/L GA₃ for 24 h. In addition, another effective soaking treatment is using 0.005 g/mL KNO₃ for 12 h. Conclusion The appropriate soaking reagent and time for the seed germination of P. grandiflorum are obtained, which could provide the guidance for seedling and artificial cultivating P. grandiflorum.

山东省高等学校科技计划项目（J11LC06）；青岛农业大学高层次人才启动基金（630909）