目的 探讨蜜环菌菌索多糖（AMP）延缓秀丽隐杆线虫衰老的作用机制。方法 以模式生物秀丽隐杆线虫作为活体模型，测定其在正常培养条件下的生存期与后代平均数量，氧化应激条件下的存活率和热休克蛋白16.2（HSP-16.2）、超氧化物歧化酶-3（SOD-3）的表达。结果 在正常培养条件下，蜜环菌菌索多糖（AMP-1、AMP-2）显著延长秀丽隐杆线虫的生存期，对其生殖力没有损害；在氧化应激条件下，显著提高秀丽隐杆线虫HSP-16.2和SOD-3的表达。结论 提高秀丽隐杆线虫的氧化应激能力可能是AMP延缓秀丽隐杆线虫衰老的作用机制。

Objective To explore the anti-aging mechanism of polysaccharide from rhizomorph of Armillaria mellea (AMP) in Caenorhabditis elegans. Methods With C. elegans as living model, the life span and the average number of offsprings were determined under normal culture conditions; The survival rate under oxidative stress and the expression of heat shock protein-16.2 (HSP-16.2) and superoxide dismutase-3 (SOD-3) were determined. Results Under the normal culture conditions, the life span of C. elegans was significantly extended by AMP-1 and AMP-2 without the damage of the reproductive capacity; The expression of HSP-16.2 and SOD-3 was increased in C. elegans under the oxidative stress. Conclusion The possible mechanism of AMP for anti-aging of C. elegans may be caused by increasing of the capacity of stress resistance.

国家自然科学基金资助项目（21077040）