目的 分析四倍体菘蓝Isatis indigotica基因组DNA甲基化水平和模式。方法 采用甲基化敏感扩增多态性（methylation sensitive amplification polymorphism，MSAP）方法，对四倍体菘蓝基因组DNA的甲基化水平和模式进行分析。结果 筛选36对MSAP选择性引物进行扩增，共得到1 733条清晰的条带。二倍体菘蓝基因组胞嘧啶甲基化水平为24.05%，四倍体菘蓝基因组胞嘧啶甲基化水平为23.70%。检测到83个（4.86%）CCGG位点在四倍体菘蓝基因组中发生了DNA甲基化模式的改变，其中37个（2.13%）位点发生了超甲基化，36个（2.08%）位点发生了去甲基化。结论 二倍体和四倍体菘蓝在CCGG位点上存在胞嘧啶甲基化状态的改变。

Objective To analyze genome cytosine methylation level and cytosine methyltion patterns of tetraploid Isatis indigotica. Methods Methylation sensitive amplification polymorphism (MSAP) approach was used in this study to analyze the genome cytosine methylation level and cytosine methyltion patterns of tetraploid I. indigotica. Results By using 36 pairs of selective primer combinations, 1 733 bands were obtained. The genome cytosine methylation levels of diploid and tetraploid I. indigotica were 24.05% and 23.70%, respectively. In all, 83 (4.86%) CCGG sites displayed cytosine methylation changes in tetraploid I. indigotica. Among these sites, 37 (2.13%) CCGG sites underwent hypermethylation changes and 36 (2.08%) CCGG sites underwent demethylation changes. Conclusion This result provides the evidence for cytosine methylation changes in CCGG sites of both diploid and tetraploid I. indigotica.

教育部高等学校博士学科点专项科研基金课题（20114220120003，20114220120005）；湖北省自然科学基金湖北省自然科学基金（2012FFB00104，2010CDB04403）；湖北省教育厅科研项目（Q20111307）