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[摘要]
目的 研究灰毡毛忍冬的主要药效,并与忍冬相关药效进行比较。方法 通过测定灰毡毛忍冬的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),研究其对6种常见致病菌的抗菌作用;采用醋酸致小鼠腹腔毛细血管通透性增加、二甲苯致小鼠耳廓肿胀、角叉菜胶致大鼠足肿胀和大鼠棉球植入致肉芽肿等实验,研究其抗炎作用;采用酵母粉致热大鼠模型,研究其解热作用;通过测定大鼠白细胞数和免疫低下小鼠免疫器官质量及碳粒廓清实验,研究其免疫作用,并与忍冬进行比较。结果 灰毡毛忍冬对6种常见致病菌具有显著的抑菌和杀菌作用,MIC为7.8~62.8 mg/mL,MBC≥250 mg/mL;灰毡毛忍冬(10 g/kg)能显著降低小鼠腹腔毛细血管通透性、大鼠足肿胀度和减少肉芽组织的增生,有抑制小鼠耳肿胀和降低发热大鼠体温的趋势,显著提高小鼠脏器指数、廓清指数(K)、吞噬指数(α),有增加大鼠白细胞数的趋势。灰毡毛忍冬与忍冬的上述药效学指标无显著差异。结论 灰毡毛忍冬具有抗菌、抗炎作用和解热的趋势,能增强动物非特异性免疫功能,作用强度与忍冬相当。
[Key word]
[Abstract]
Objective To study the main efficacies of Lonicera macranthoides by comparing with the efficacy of L. japonica. Methods The measurements of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were used to study the antibacterial effect; The increasing permeability of abdominal capillary in mice, the ear edema of mice induced by xylene, the paw edema model of rats induced by carrageenan, and the granuloma model of mice induced by embedding cotton pellet were obtained to study the anti-inflammatory effects; The fevered rat model induced by yeast powder was used to study the antipyretic action; The carbon clearance experiment, weight determination of immune organ in immuno-compromised mice, and leukocyte determination of rats were used to study the immunoregulatory function; The results were compared with those of L. japonica. Results L. macranthoides showed the significant antibacterial effect on six kinds of pathogenic bacteria, with MIC of 7.8—62.8 mg/mL and MBC ≥ 250 mg/mL. L. macranthoides (10 g/kg) could considerably decrease the permeability of abdominal capillary, paw edema, and granuloma, and reduce the ear edema and body temperature of fever rats to some degree. It could increase the leukocyte numbers, the index of immune organ, clearance index (K), and phagocytic index (α). However, there was no great difference by comparative analysis between L. macranthoides and L. japonica (P > 0.05). Conclusion L. macranthoides has the antibacterial, anti-inflammatory, antipyretic, and immunoregulatory functions. Moreover, the drug efficacies are almost equal compared with L. japonica .
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[基金项目]
国家科技计划课题专项(2006BACD1A16);科技部富民强县专项行动计划项目(国科发农[2008]471号)