[关键词]
[摘要]
目的 通过建立RP-HPLC法测定白芍-甘草药对中12种有效成分,并用此方法探讨有效成分随白芍-甘草配比变化的溶出规律。方法 采用Dikma Technologies-C18色谱柱(200 mm×4.6 mm,5 μm),流动相为乙腈(A)-0.1%磷酸水溶液(B),梯度洗脱:0~5 min,5%~10% A;5~10 min,10%~12% A;10~15 min,12%~14% A;15~20 min,14%~16% A;20~25 min,16%~18% A;25~30 min,18%~20% A;30~40 min,20%~25% A;40~50 min,25%~40% A;50~62 min,40%~55% A;62~72 min,55%~70% A;72~85 min,70%~55% A;85~95 min,55%~5% A,体积流量1.0 mL/min,检测波长为267 nm(0~13 min)、258 nm(13~17 min)、230 nm(17~27 min)、276 nm(27~32 min)、230 nm(32~42 min)、360 nm(42~46 min)、276 nm(46~50 min)、230 nm(50~53 min)、275 nm(53~55 min)、250 nm(55~95 min),柱温30 ℃。结果 在所观察的白芍-甘草的9个配伍比例(0∶1、0.3∶1、0.6∶1、1∶1、2∶1、3∶1、4∶1、5∶1、1∶0)中,以1∶1、3∶1组有效成分溶出量最高,另外0.6∶1组也较高。结论 以现代研究方法证实了张仲景《伤寒论》芍甘汤中配伍比例1∶1的科学性,也进一步从有效成分上证实了现代临床经典比例3∶1配伍的合理性。
[Key word]
[Abstract]
Objective To establish the RP-HPLC method for the determination of twelve active components in the combination of Paeoniae Radix Alba (PRA) and Glycyrrhizae Radix et Rhizoma (GRR) and to further analyze the principle of the active component dissolution with the proportion changing. Methods The separation was performed on a Dikma Technologies-C18 BDS (200 mm × 4.6 mm, 5 μm) column with the gradient elution: 0—5 min, 5%—10% A; 5—10 min, 10%—12% A; 10—15 min, 12%—14% A; 15—20 min, 14%—16% A; 20—25 min, 16%—18% A; 25—30 min, 18%—20% A; 30—40 min, 20%—25% A; 40—50 min, 25%—40% A; 50—62 min, 40%—55% A; 62—72 min, 55%—70% A; 72—85 min, 70%—55% A; 85—95 min, 55%—5% A using acetonitrile (A)-water of 0.1% phosphoric acid (B) at the flow rate of 1.0 mL/min; Detection wavelength was 267 nm (0—13 min), 258 nm (13—17 min), 230 nm (17—27 min), 276 nm (27—32 min), 230 nm (32—42 min), 360 nm (42—46 min), 276 nm (46—50 min), 230 nm (50—53 min), 275 nm (53—55 min), and 250 nm (55—95 min); The column temperature was 30 ℃. Results Among the observed nine compatibility proportions (PRA-GRR as 0∶1, 0.3∶1, 0.6∶1, 1∶1, 2∶1, 3∶1, 4∶1, 5∶1, and 1∶0), the active component dissolution amounts in 1∶1 and 3∶1 groups were the highest, and 0.6∶1 group also showed higher dissolution amount. Conclusion The scientificity of 1∶1 combination of PRA and GRR in Treatise on Cold Damage Diseases by ZHANG Zhong-jing has been proved using modern research method and also the rationality of the modern classical 3∶1 ratio has been further confirmed in clinic from the active components aspect.
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[基金项目]
辽宁省教育厅课题“中药材品质评价体系研究”(2008S145)