[关键词]
[摘要]
目的 研究芫花Daphne genkwa中的瑞香烷型二萜原酸酯类成分及其肿瘤细胞毒活性。方法 采用色谱分离技术进行分离和纯化,并根据谱学数据确定化合物的结构,采用MTT法评价化合物对NCI-H1975肺癌细胞株和SK-BR-3乳腺癌细胞株的细胞毒活性。结果 从芫花中分离得到6个化合物,分别鉴定为异芫花酯甲(1)、芫花酯甲(2)、芫花酯丁(3)、芫花酯庚(4)、异芫花酯乙(5)、芫花酯乙(6)。结论 化合物1为新化合物。化合物1~6对人乳腺癌SK-BR-3细胞株显示了较强的细胞毒活性,IC50值分别为217.1、172.6、170.2、809.4、83.7、61.6 nmol/L,而对肺癌NCI-H1975细胞株未显示细胞毒活性。
[Key word]
[Abstract]
Abstract: Objective To study daphnane diterpene esters from the flower buds of Daphne genkwa and to evaluate their cytotoxicity against tumor cells. Methods The compounds were isolated and purified by means of chromatographic techniques and their structures were identified on the basis of spectral features. Cytotoxic activity against NCI-H1975 lung cancer cells and SK-BR-3 breast cancer cells were assessed for all the compounds by MTT method. Results Six compounds were isolated and identified as isoyuanhuacin (1), yuanhuacin (2), yuanhuatin (3), yuanhuagin (4), isoyuanhuadin (5), and yuanhuadin (6). Conclusion Compound 1 is a new compound. Compounds 1—6 exhibit the strong cytotoxicity on SK-BR-3 human breast cancer cells with the IC50 values of 217.1, 172.6, 170.2, 809.4, 83.7, and 61.6 nmol/L, respectively, but show no effect on NCI-H1975 human lung cancer cells.
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[基金项目]
国家重大新药创新专项(2011ZX09401-009)