[关键词]
[摘要]
目的 利用近红外透反射光谱技术,对复方苦参注射液渗漉液中多组分的量进行快速测定。方法 采用氧化槐果碱、氧化苦参碱的HPLC测定值,总糖的苯酚-硫酸法测定值及固体总量的烘烤法测定值为对照值,用偏最小二乘法建立近红外光谱与对照值之间的校正模型,并对未知样品进行定量预测。结果 校正模型对氧化槐果碱、氧化苦参碱、总糖和固体总量4种组分的交叉验证均方根误差分别为43.5、135.4、1 255.7 mg/L和150.7 mg/mL,相关系数分别为0.926 6、0.954 6、0.953 9和0.974 1。经外部验证,预测均方根误差分别为32.5、191.7、1 461.9 mg/L和164.0 mg/mL。结论 本方法方便、准确、可靠,可用于中药渗漉过程的快速分析。
[Key word]
[Abstract]
Objective To develop a fast analytical method for determining the multiple components in the percolation of Fufang Kushen Injection with near infrared transflective spectroscopy. Methods The contents of oxysophocarpine and oxymalrine by HPLC, total sugar by phenol-sulfuric acid method, and total solids by baking method were used as reference values. Multivariate calibration models based on PLS algorithm were developed to correlate the near infrared spectroscopy and the corresponding values, and the unknown samples were quantitively determined. Results The root mean square errors of cross-validation in calibration model for oxysophocarpine, oxymalrine, total sugar, and total solids were 43.5, 135.4, 1 255.7 mg/L, and 150.7 mg/mL, respectively, and the correlation coefficients were 0.926 6, 0.954 6, 0.953 9, and 0.974 1, respectively. External validation with external samples indicated that the root mean square errors of prediction were 32.5, 191.7, 1 461.9 mg/L, and 164.0 mg/mL, respectively. Conclusion The presented method is fast, accurate, and reliable, which could be used as a process analyzing method for the percolation process of Chinese materia medica.
[中图分类号]
[基金项目]
国家“重大新药创制”科技重大专项(2008ZX09202-009)