[关键词]
[摘要]
目的 从UPLC特征指纹图谱和6-姜酚、8-姜酚、10-姜酚量的角度,选择炮姜的最佳炮制温度和炮制时间。方法 采用砂烫法,选取180、185、190、195、200 ℃,每个温度下分别炒制6、7、8 min,共得到15份样品;采用超高效液相色谱(UPLC)法分析各样品指纹图谱相似度;同时采用UPLC法测定不同炮制品中6-姜酚、8-姜酚、10-姜酚的量。结果 195 ℃、7 min和195 ℃、8 min 2份样品的相似度最大,分别为0.995和0.994;6-姜酚、8-姜酚、10-姜酚的总量最低,分别为5.792、5.412 mg/g。结论 综合指纹图谱相似度和6-姜酚、8-姜酚、10-姜酚量的测定结果以及炮制品外观,确定炮姜砂烫炮制以195 ℃、7 min为宜。
[Key word]
[Abstract]
Objective To optimize the processing temperature and time of Zingiberis Rhizoma Praeparata (ZRP) according to ultra- high performance liquid chromatography (UPLC) fingerprints and the contents of 6-gingerol, 8-gingerol, and 10-gingerol. Methods Samples were treated by stir-baking in sand method at 180, 185, 190, 195, and 200 ℃, stirred for 6, 7, and 8 min at each temperature, respectively. Then the fingerprint similarity of the samples and the contents of 6-gingerol, 8-gingerol, and 10-gingerol were determined by UPLC method. Results The similarities of the two samples at 195 ℃ for 7 min and for 8 min were the highest, 0.995 and 0.994, while the contents of 6-gingerol, 8-gingerol, and 10-gingerol of the two samples were the lowest, 5.792 and 5.412 mg/g, respectively. Conclusion According to the similarities of the fingerprint, the contents of 6-gingerol, 8-gingerol, 10-gingerol, and the apperance of the processed products, ZRP should be better processed by stir-baking in sand method at 195 ℃ for 7 min.
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[基金项目]
国家自然科学基金资助项目(81102811);国家中医药重点学科临床中药学建设项目(国中医药人教发 [2012]32号);安徽省自然科学基金资助项目(10040606Q40)