[关键词]
[摘要]
目的从分子水平鉴别濒危药用植物毛黄堇和石生黄堇的差异。方法分别从毛黄堇和石生黄堇的叶片中提取DNA,以核基因组通用引物为引物进行扩增,扩增产物经纯化后对PCR产物采用直接测序法进行测序。结果获得ITS及5.8 S rDNA完全序列,分别为毛黄堇561 bp、石生黄堇552 bp;二者的ITS序列差异呈显著性,其中ITS1差异性达16.28%,ITS2差异性达21.21%。结论 ITS序列可有效地鉴别毛黄堇和石生黄堇,并可广泛应用于中药材的鉴别。
[Key word]
[Abstract]
Objective To identify the endangered medicinal plants Corydalis tomentellaand C. saxicolaat molecular level. Methods DNA was extracted from the leaves of C. tomentellaand C. saxicolaand amplified by common primers in nuclear genome. The amplification products were sequenced using direct sequencing method after purification. Results The rDNA full-length sequences of ITS and 5.8S, i.e.561 and 552 bp for C. tomentellaand C. saxicola, were obtained, respectively. Significant differences were found between the two ITS sequences, and differences were 16.28% and 21.21% for ITS1 and ITS2, respectively. Conclusion ITS sequence could be used for the identification of C. tomentellaand C. saxicola, and also be widely used for the identification of Chinese materia medica.
[中图分类号]
[基金项目]
科技部“十一五”支撑计划项目(2006BAC01A16);重庆市中医药重点攻关计划项目(20090116,201002166)