[关键词]
[摘要]
目的 考察影响洋川芎内酯A和洋川芎内酯I稳定性的因素,分离和鉴定其室温自然光照条件下放置2个月后的降解产物,并推测其可能降解途径。方法 半制备HPLC分离制备降解产物,UV、MS和NMR鉴定结构。结果 氧是影响洋川芎内酯A和洋川芎内酯I稳定性的主要因素,室温自然光照2个月后,洋川芎内酯A通过脱氢反应完全转化为丁基苯酞;洋川芎内酯I通过异构化反应部分转化为其同分异构体 (E)-6, 7-反式-双羟基藁本内酯。结论 洋川芎内酯A和洋川芎内酯I要尽量保存在低温、避光和无氧环境中,以保持其稳定性。
[Key word]
[Abstract]
Objective To investigate the factors influencing the stability of senkyunolide A and senkyunolide I, then to isolate and identify the degradation products stored at room temperature under direct sunlight for 2 months. The possible degradation pathways were proposed. Methods Semi-preparative HPLC was employed to isolate the degradation products. UV, MS, and NMR were used for the structure elucidation. Results Oxygen is the major factor influencing the stability of senkyunolide A and sekyunolide I. Under the conditions mentioned above, senkyunolide A was completely transferred into butylphthalide through dehydrogenation, while senkyunolide I was partly transferred into its isomer (E)-6, 7-transdihydroxyligustilide through isomerization after direct sunlight for 2 months. Conclusion To keep their stability, senkyunolide A and sekyunolide I should be stored at low temperature condition without light and oxygen.
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[基金项目]
国家自然科学基金资助项目(30973873)