目的观察大柴胡颗粒对胆色素结石豚鼠的保护作用。方法将豚鼠随机分为对照组,模型组,大柴胡颗粒高、中、低剂量(4.4、2.2、1.1 g/kg)组和熊去氧胆酸(50 mg/kg)组。采用饲料法复制胆色素结石豚鼠模型,观察大柴胡颗粒对胆色素结石豚鼠的成石率、血清天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、碱性磷酸酶(AKP)、谷氨酰基转移酶(γ-GT)、总胆红素(TB)、1分钟胆红素水平、血浆和胆囊组织胃动素和生长抑素(SS)水平,以及胆汁内总胆酸(TBA)、磷脂、总胆固醇(TC)、黏蛋白量的影响;肝脏和胆囊组织进行HE染色,观察其组织病变情况。结果大柴胡颗粒中剂量组可显著降低结石成石率;其3个剂量组均可降低胆囊SS水平;其中、高剂量组可显著升高血浆和胆囊胃动素水平,降低血清γ-GT、1分钟胆红素水平和胆汁黏蛋白的量;其高剂量组可显著降低血清AST和TB、血浆SS和胆汁中TC水平,升高胆汁中TBA的量;而低剂量组可显著升高胆汁中磷脂的量,与模型组比较差异显著(P<0.05、0.01)。组织病理学观察表明大柴胡颗粒可显著改善胆囊上皮增生,减轻组织水肿、炎细胞浸润、肝脏脂肪变性、汇管区炎细胞浸润和纤维组织增生。结论大柴胡颗粒对胆色素结石豚鼠具有一定的保护作用,该作用可能与其减少肝胆细胞损伤、调节胆汁成分、促进胆汁分泌等机制相关。

Objective To observe the protection of Dacaihu Granules (DG) on guinea pigs with bile pigment stones. Methods Guinea pigs were randomly divided into six groups, such as control, model, DG high-, mid-, low-dose (4.4, 2.2, and 1.1 g/kg), and ursodeoxycholic acid (50 mg/kg) groups. The guinea pigs with bile pigment stones were established by fodder adjusting. With the interference of DGthe stone-forming rate, the levels of aspartate transaminase (AST), alanine transarninase (ALT), alkaline phosphatase (AKP), y-glutamyl transferase (y-GT), total bilirubin (TB), one minute bilirubin, motilin and somatostatin (SS) in plasma and gallbladder tissue were observed, and the content of total bile acids (TBA), phospholipids, total cholesterol (TC), and mucin in bile were detected; HE staining was carried out and tissue lesions were observed in the liver and gallbladder tissue. Results Three doses of DG could decrease the concentration of SS in gallbladder tissue. High- and mid-dose of DG could increase the content of motilin in gallbladder and plasma, and decrease the concentration of y-GT, one minute bilirubin in serum, and mucin in bile; High-dose of DG could decrease AS工TB, and SS levels in plasma and TC in bile, and increase the content of TBA in bile; Mid-dose of DG could decrease the stone-forming rate and low-dose of DG could significantly increase the content of phospholipids in the bile campared with model group in obvious difference (P<0.05, 0.01). In addition, the histological examination indicated that DG could markedly improve gallbladder epithelial hyperplasia and reduce tissue edema, inflammatory cell infiltration, hepatic steatosis, portal area inflammatory cell infiltration, and fibrous tissue proliferation. Conclusion DG displays the protection on guinea pigs with bile pigment stones to some extent, and the effect might involve with protecting liver and gallbladder cells, adjusting bile composition, and improving bile secretion.

江苏省科技成果转化专项课题(BA2010094)