目的 研究葛根芩连配方颗粒（简称葛根芩连颗粒）在大肠湿热证模型大鼠尿液中的代谢产物。方法 采用综合因素（高糖和高脂饮食＋高温和高湿环境＋肠产毒性大肠杆菌致病因子）制备大肠湿热证大鼠模型；利用HPLC-DAD-MS技术对大鼠ig葛根芩连颗粒后尿液中代谢产物进行分析，检测波长272 nm；流动相A为0.1%甲酸水溶液，流动相B为乙腈-甲醇（6∶4），梯度洗脱。结果 从给予葛根芩连颗粒的大鼠尿液中发现并鉴定了5种主要代谢产物，分别为葛根素、异甘草素、甘草素、汉黄芩素、千层纸素A，其中葛根素来自葛根，汉黄芩素与千层纸素A来自黄芩，异甘草素与甘草素来自甘草。结论 在大肠湿热证模型大鼠尿液中，葛根芩连颗粒中黄芩与甘草的代谢产物主要以苷元为主，葛根中的葛根素主要以原形排出。

Objective To study the metabolites of Gegen Qinlian Dispensing Granule (GQDG) in urine of rats model with large intestinal damp-heat syndrome. Methods The animal model with large intestine damp-heat syndrome was developed by multiple factors (high glucose and high fat diet, environment under high humidity and high temperature, and pathogenic biological factors of enterotoxigenic Escherichia coli). The metabolites in urine of rats after ig administration of GQDG were analyzed by HPLC-DAD-MS. The detection wavelength was 272 nm; Mobile phase was 0.1% formic acid-water and acetonitrile-methanol (6:4). Results Five components were identified as puerarin (from Puerariae Radix), isoliquiritigenin, liquiritigenin (from Glycyrrhizae Preparata Radix), wogonin, and oroxylin A (from Scutellariae Radix). Conclusion Metabolites of Scutellariae Radix and Glycyrrhizae Preparata Radix in GQDG in urine of model rats with large intestinal damp-heat syndrome are mainly aglycone, and the metabolites of puerarin in Puerariae Radix are unchanged and mainly excreted by prototype via urine.

国家自然科学基金资助项目（30901995）