目的 探讨白花丹参总酚酸提取物对血栓闭塞性脉管炎模型大鼠的影响，为其临床用于治疗血栓闭塞性脉管炎提供科学依据。方法 采用LC-UV-MS法对白花丹参总酚酸提取物中的主要成分进行定性分析。Wistar大鼠随机分为假手术组，模型组，白花丹参总酚酸提取物高、中、低剂量（5.63、2.82、1.41 g/kg）组，通塞脉片（0.66 g/kg）阳性对照组。大鼠股动脉注射月桂酸溶液制备血栓闭塞性脉管炎模型后第2天ig给药，每天给药1次，连续给药1周，观察用药前后大鼠的体征、血管平滑肌和纤维组织等病理变化，以及血清中血栓素B₂（TXB₂）、6-酮前列腺素1α（6-k-PGF_1α）、内皮素（ET）水平。结果 白花丹参总酚酸提取物明显减轻血栓闭塞性脉管炎大鼠患肢病变程度和范围，减轻炎症反应，减少血管内血栓形成，降低血清中TXB₂、ET水平，升高6-k-PGF_1α水平。结论 白花丹参总酚酸提取物对血栓闭塞性脉管炎大鼠具有较好的治疗作用，其作用机制与其抗氧化、减轻血管壁炎症反应有关。

Objective To study the effect of total salvianolic acid from Salvia miltiorrhiza f. alba (TSAS) on rat models with thromboangiitis obliterans (TAO) and provide basis for treatment of this disease. Methods Principal components analysis in TSAS was carried out by LC-UV-MS. Wistar rats were randomly divided into six groups, such as Sham, model, TSAS high-, mid-, and low-dose (5.63, 2.82, and 1.41 g/kg) groups, as well as Tongsemai Tablets (0.66 g/kg) as positive group. TAO model was prepared by iv injecting lauric acid solution into the femoral artery of rats and on the second day the rats were ig administrated with TSAS and Tongsemai Tablets, respectively once a day for a week. The changes of physical signs and pathologic grading of vascular smooth muscle and fibrous tissue were observed and the levels of thromboxane B₂ (TXB₂), endothelin (ET), and 6-keto-prostaglandin F_lα (6-k-PGF_1α) in serum were detected. Results TSAS significantly reduced the degree and scope of limb lesion in animals of treating group, with the inflammatory reaction and number of thrombus formatron inhibited, decreased the levels of TXB₂ and ET, while increased the level of 6-k-PGF_1α in serum. Conclusion TSAS has the significant therapeutic effect on the experimental TAO prepared among rats. The possible mechanisms for this effect may be achieved by improving anti-oxidative function of vessel endothelia and inhibiting the inflammatory reaction in vascular wall.

山东省自然科学基金资助项目（2009ZRB019UX）