目的 研究毛冬青Ilex pubescens叶的化学成分。方法 采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及半制备高效液相色谱法进行分离纯化，并通过理化性质与光谱分析法鉴定化合物的结构。结果 从毛冬青叶70%乙醇提取物中分离鉴定了16个化合物，分别为大豆苷元（1）、染料木苷（2）、山柰酚-3-O-β-龙胆二糖苷（3）、山柰酚-3-O-β-刺槐双糖苷（4）、山柰酚-3-O-β-半乳糖苷（5）、槲皮素-3-O-β-龙胆二糖苷（6）、3β, 19α-二羟基齐墩果-12-烯-24, 28-二酸-28-O-β-D-吡喃葡萄糖苷（7）、毛冬青皂苷A₁（8）、毛冬青素A（9）、2-羟甲基-3-咖啡酰氧-1-丁烯-4-O-β-D-吡喃葡萄糖苷（10）、2-咖啡酰甲基-3-羟基-1-丁烯-4-O-β-D-吡喃葡萄糖苷（11）、3, 4-O-二咖啡酰基奎宁酸（12）、3, 5-O-二咖啡酰基奎宁酸（13）、1, 5-O-二咖啡酰基奎宁酸（14）、4, 5-O-二咖啡酰基奎宁酸（15）、2-苯乙基-O-α-L-阿拉伯糖基 (1→6)-O-β-D-吡喃葡萄糖苷（16）。结论 化合物1～6、12～16为首次从该植物中分离得到。

Objective To study the chemical constituents from the leaves of Ilex pubescens. Methods The chemical constituents from the leaves of I. pubescens were isolated and purified by silica column chromatography, ODS, Sephadex LH-20 column chromatography, and semi-preparation HPLC. Their structures were elucidated by chemical and spectral analyses. Results Sixteen compounds were obtained from 70% ethanol extract and identified as daidzein (1), genistin (2), kaempferol-3-O-β-gentiobioside (3), kaempferol-3-O-β-robinobinoside (4), kaempferol-3-O-β-galactopyranoside (5), quercetin-3-O-β-gentiobioside (6), 3β, 19α-di- hydroxyolean-12-ene-24, 28-dioic-28-O-β-D-glucopyranoisde (7), ilexsaponin A₁ (8), ilexgenin A (9), 2-hydroxymethyl-3-caffeoyloxyl- 1-butene-4-O-β-D-glucopyranoside (10), 2-caffeoyloxymethyl-3-hydroxyl-1-butene-4-O-β-D-glucopyranoside (11), 3, 4-O-diocaffeoyl- quinic acid (12), 3, 5-O-diocaffeoylquinic acid (13), 1, 5-O-diocaffeoylquinic acid (14), 4, 5-O-diocaffeoylquinic acid (15), and 2-phenylethyl- O-α-L-arabinopyranosyl (1→6)-O-β-D-glucopyranoside (16). Conclusion Compounds 1—6 and 12—16 are isolated from this plant for the first time.

国家自然科学基金资助项目（30672608）；国家重大新药创制专项资助项目（2009ZX09311-004）