[关键词]
[摘要]
目的 研究艾迪注射液的化学成分。方法 利用反相半制备液相色谱、Sephadex LH-20凝胶柱色谱等方法分离纯化,并通过光谱数据鉴定化合物结构。结果 分离得到22个化合物,分别鉴定为3-O-(3′, 4′-二乙酰氧基)-β-D-吡喃木糖基-6- O-β-D-吡喃葡萄糖基-环黄芪醇(1)、黄芪甲苷(2)、黄芪皂苷II(3)、黄芪皂苷I(4)、异黄芪皂苷I(5)、乙酰黄芪皂苷I(6)、人参皂苷Re(7)、人参皂苷Rf(8)、人参皂苷Rg1(9)、人参皂苷Rb3(10)、三七皂苷R4(11)、人参皂苷Rb1(12)、人参皂苷Rc(13)、人参皂苷Rb2(14)、人参皂苷Rd(15)、丝瓜苷H(16)、3-O-β-D-吡喃葡萄糖基 (1→4)-β-D-吡喃葡萄糖基 (1→3)-α-L-吡喃鼠李糖 (1→2)-α-L-吡喃阿拉伯糖-齐墩果酸-28-O-α-L-吡喃鼠李糖 (1→4)-β-D-吡喃葡萄糖 (1→6)-β-D-吡喃葡萄糖酯苷(17)、3-O-β-D-吡喃葡萄糖基 (1→3)-α-L-吡喃鼠李糖 [β-D-吡喃葡萄糖基-(1→4)]-(1→2)-α-L-吡喃阿拉伯糖-齐墩果酸-28-O-α-L-吡喃鼠李糖 (1→4)-β-D-吡喃葡萄糖 (1→6)-β-D-吡喃葡萄糖酯苷(18)、紫丁香苷(19)、刺五加皂苷E(20)、4-(1, 2, 3-三羟基丙基)-2, 6-二甲氧基苯-1-O-β-D-葡萄糖苷(21)、松柏苷(22)。结论 经LC-MS分析检测,化合物1~6为黄芪中的化学成分,化合物7~18为人参中的化学成分,化合物19~22为刺五加中的化学成分,其中化合物1为新化合物,命名为新黄芪皂苷I。
[Key word]
[Abstract]
Objective To investigate the chemical constituents from Aidi Injection. Methods The chemical constituents were isolated by chromatography on Sephadex LH-20 gel columns and reverse phase semi-preparation HPLC repeatedly, and their structures were identified by spectral data. Results Twenty-two compounds were isolated and identified as 3-O-(3′, 4′-diacetyl)-β-D- xylopyranosyl-6-O-β-D-glucopyranosyl-cycloastragenol (1), astragaloside IV (2), astragaloside II (3), astragaloside I (4), isoastragaloside I (5), acetylastragaloside I (6), ginsenosid-Re (7), ginsenoside-Rf (8), ginsenoside-Rg1 (9), ginsenoside-Rb3 (10), notoginsenoside-R4 (11), ginsenoside-Rb1 (12), ginsenoside-Rc (13), ginsenoside-Rb2 (14), ginsenoside-Rd (15), lucyoside H (16), 3-O-β-D-glucopyranosyl (1→4)-β-D-glucopyranosyl (1→3)-α-L-rhamnopyranosyl (1→2)-α-L-arabinopyranosyl oleanolic acid 28-O- α-L-rhamnopyranosyl (1→4)-β-D-glucopyranosyl (1→6)-β-D-glucopyranoside (17), 3-O-β-D-glucopyranosyl (1→3)-α-L-rhamno- pyranosyl [β-D-glucopyranosyl-(1→4)]-(1→2)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-arabinopyranosyl (1→4)-β-D-gluco- pyranosyl (1→6)-β-D-glucopyranoside (18), syringin (19), elentheroside E (20), 4-(1, 2, 3-trihydroxypropyl)-2, 6-dimethoxyphenyl- 1-O-β-D-glucopyranoside (21), and coniferin (22). Conclusion LC-MS analysis shows that compounds 1—6 are originated from Astragalus membranceus, compounds 7—18 from Panax ginseng, and compounds 19—22 from Acanthopanacis senticosi. Compound 1 is a new compound named neoastragaloside I.
[中图分类号]
[基金项目]
重大新药创制国家科技重大专项资助项目(2009ZX09308-003,2011ZX09201-201-16)