目的探索广西草珊瑚Sarcandra glabra种质资源的遗传多样性,为草珊瑚及其他中药种质资源保护、亲缘关系研究、品种选育和引种栽培提供分子生物学依据。方法利用AFLP标记对来自广西不同产地的18份草珊瑚开展遗传多样性分析,采用NTSYS软件进行聚类和主坐标分析。目的从64对AFLP引物组合中筛选出8对用于扩增,共获得319条清晰可辨条带,其中多态性条带251条,多态性位点百分率为78.68%。聚类和主坐标分析表明广西草珊瑚种质资源的遗传基础存在一定的多样性,但遗传相似性系数(0.630 3～0.836 6)较大,遗传距离较近。结论广西草珊瑚种质资源的遗传多样性偏低,应该尽快采取相应措施,对草珊瑚资源进行合理保护。

Objective To evaluate the genetic diversity of germphlasm resources for Sarcandra glabra and provide biological reference for germplasm resources protection, genetic relationship analysis, breeding, and introduction and cultivation of S. glabra and other Chinese herbal medicinesMethods Amplified fragment length polymorphism (AFLP) marker was developed to analyze the genetic diversity among 18 samples of S. glabra from various habitats in Guangxi region, and cluster analysis and principal coordinate analysis were revealed by the NTSYS softwareResults Eight out of sixty-four AFLP primer combinations selected were used for amplification and a total of 319 unambiguous bands were obtained, among which 251 (PPB=78.68%) were polymorphic. The results of cluster analysis and principal coordinate analysis showed that there was a certain genetic basis of diversity in S. glabra. The genetic similarity coefficients (0.630 3-0.836 6) were bigger while the genetic distance was closerConclusion The genetic diversity of germplasm resources for S. glabra in Guangxi region is not abundant and protection strategies should be carried out on S. glabra.

广西科学研究与技术开发计划项目资助﹙桂科攻1099063-13﹚