目的 分析黄柏炭制前后化学成分的变化情况，为黄柏炭炮制原理的阐明提供依据。方法 按照《中国药典》2010年版方法炮制黄柏炭，采用RPLC/Q-TOF-MS技术对生黄柏及黄柏炭的提取样品进行检测，找出炮制前后具有显著性差异的物质。结果 共找出21种化学成分在炮制前后具有显著性差异，其中炮制后新生成的物质有5种，质量分数增加的物质有8种，减少的物质有8种。结论 采用RPLC/Q-TOF-MS法进行中药炮制研究具有方法灵敏度高，结果准确可靠的优点，该法能够全面地反映出中药在炮制过程中多种化学成分的变化。

Objective To analyze the changes of chemical constituents in Phellodendri Chinensis Cortex before and after charing and provide bases for clarifying the processing principles of Phellodendri Chinensis Cortex. Methods Phellodendri Chinensis Cortex was processed according to Chinese Pharmacopoeia 2010 and the extracts of unprocessed and processed Phellodendri Chinensis Cortex were detected by using RPLC/Q-TOF-MS. The constituents with significant differences between the unprocessed and processed Phellodendri Chinensis Cortex were identified. Results The results showed that a total of 21 chemical constituents had significant differences before and after processing including five new constituents, eight constituents with content increasing, and eight constituents with content decreasing. Conclusion The RPLC/Q-TOF-MS method proves to have a high sensitivity and the result is accurate and reliable. The method could fully reflect the changes of multiple chemical constituents of Chinese materia medica in the course of processing.

国家自然科学基金资助项目（30801540）