[关键词]
[摘要]
目的建立HPLC同时测定凉血通瘀颗粒中芍药苷、丹皮酚、芦荟大黄素、α-细辛醚、大黄素、大黄酚、大黄素甲醚7种成分的方法。方法色谱柱为Lichrospher C18柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)-0.1%磷酸水溶液(B),梯度洗脱:0~18 min,15%A,体积流量0.8 mL/min;18~20 min,15%~42%A,体积流量0.8 mL/min;20~54 min,42%A,体积流量0.8 mL/min;54~56 min,42%~70%A,体积流量1.0 mL/min;56~80 min,70%A,体积流量1.0 mL/min;检测波长254 nm,柱温25℃。结果 7种成分均能达到基线分离,各成分均有较宽的线性范围和良好的线性关系,加样回收率在95%~105%。结论本法准确、灵敏、可靠、重复性好,可用于凉血通瘀颗粒的质量控制。
[Key word]
[Abstract]
ObjectiveTo develop an HPLC method for simultaneous determination of paeoniflorin, paeonol, aloeemodin, α-asarone, emodin, chrysophanol, and physcion in Liangxue Tongyu Granule.Methods The chromatographic separation was achieved on an Lichrospher C 18 (250 mm × 4.6 mm, 5 μm) column with acetonitrile (A) - 0.1% phosphoric acid (B) as mobile phases for gradient elution, 0—18 min, 15% A at the flow rate of 0.8 mL/min; 18—20 min 15%—42% A at the flow rate of 0.8 mL/min; 20—54 min, 42% A at the the flow rate of 0.8 mL/min; 54—56 min, 42%—70% A at the flow rate of 1.0 mL/min; and 56—80 min, 70% A at the flow rate of 1.0 mL/min. The column temperature was 25 ℃ and the detection wavelength was set at 254 nm. Results The results showed that seven active components were well separated and showed good linearity. The average recoveries were between 95%—105%. Conclusion The method is accurate, sensitive, credible, and repeatable. It can be applied to the quality control of Liangxue Tongyu Granule.
[中图分类号]
[基金项目]
江苏高校优势学科建设工程资助项目(2011-2015);江苏省科技厅科技支撑计划(BE2011849);江苏省“六大人才高峰”项目(2009);江苏省“青蓝工程”科技创新团队支持计划[苏教师(2008)30号];南京中医药大学科技创新风险基金项目计划(CX200904)