目的研究尖齿臭茉莉Clerodendrum lindleyi根和茎的化学成分及其体外自由基清除活性。方法采用多种柱色谱技术进行分离纯化,根据化合物的理化性质和波谱数据进行结构鉴定;并采用DPPH法对分得的化合物进行体外自由基清除活性测试。结果从尖齿臭茉莉中分离得到13个化合物,分别鉴定为白桦脂酸(1)、单乙酰马蒂罗苷(2)、4″-O-乙酰马蒂罗苷(3)、赪桐苷A(4)、3″,4″-O-乙酰马蒂罗苷(5)、洋丁香苷(6)、木栓酮(7)、粘霉烯醇(8)、蒲公英萜醇(9)、钩大青酮(10)、海通酮E(11)、(24S)-乙基胆甾-5,22,25-三烯-3β-醇(12)、hedyotol C 4″-O-β-D-glucopyranoside(13)。化合物1～6从根中分离得到,7～13从茎中分离得到。化合物1～12显示了不同程度的DPPH清除活性。结论化合物1～13均为首次从该植物中分离得到,化合物13为首次从该属植物中分离得到。分离得到的苯乙醇苷类、萜类及甾醇类成分均具有一定的DPPH清除作用,其中苯乙醇苷类成分4″-O-乙酰马蒂罗苷、单乙酰马蒂罗苷和洋丁香苷活性最为显著。

Objective To study the chemical constituents from the roots and stems of Clerodendrum lindleyi and their in vitro activities of DPPH free radical scavenging.Methods The constituents were isolated and purified by various column chromatographic techniques and their structures were identified on the basis of their physicochemical and spectral data. Free radical scavenging activity of the isolated constituents was investigated in vitro using the DPPH assay. ResultsThirteen constituents were isolated from C. lindleyi and identified as betulinic acid (1), monoacetylmartinoside (2), 4″-O-acetylmartynoside (3), clerodenoside A (4), 3″, 4″-O-acetylmartynoside (5), acteoside (6), friedelin (7), glutinol (8), taraxerol (9), uncinatone (10), mandarone E (11), (24S)-24- ethylcholesta-5, 22, 25-trien-3β-ol (12), and hedyotol C 4″-O-β-D-glucopyranoside (13). Compounds1-6 were obtained from the roots and7-13were from the stems. Bioassay indicated that compounds 1-12 had different free radical scavenging activities. Conclusion Compounds1-13are all obtained from C. lindleyi for the first time. Compound 13is obtained from the plants in Clerodendrum Linn. for the first time. Phenylethanoid glycosides, diterpenoids, triterpenoids, and sterol obtained show different free radical scavenging activities. Phenylethanoid glycosides 4″-O-acetylmartynoside, monoacetylmartinoside, and acteoside exhibit the most significant activity.

中山市第二批产学研结合专项资金项目(2011CXY004);中国博士后科学基金资助项目(2011M501368)