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[摘要]
目的研究反柄紫芝Ganoderma cochlear子实体的化学成分。方法利用硅胶和凝胶柱色谱及半制备HPLC进行分离纯化,采用多种谱学技术(1D、2D NMR)并根据理化常数鉴定结构。结果分离并鉴定了5个化合物,分别为拱状灵芝素G(1)、拱状灵芝素H(2)、麦角甾醇(3)、5α,8α-环二氧-6,22-麦角甾二烯-3β-醇(4)和(22E,24R)-7,22-麦角甾二烯-3β-醇(5)。结论化合物1和2为新化合物,其余化合物均为首次从灵芝属真菌中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents from the sporophore of Ganoderma cochlear. Methods The methanol extracts of G. cochlear were treated by repeated silica gel, Sephadex LH-20 column chromatography and semi-preparative HPLC. Structures were identified by physicochemical data and 1D and 2D NMR technology. Results From the sporophore of G. cochlear, two new 3, 4-seco-trinorlanostane triterpenoids, named fornicatin G (1) and fornicatin H (2), together with three known ergostane sterols, named ergosterol (3), 5α, 8α-epi-dioxyergosta-6, 22-dien-3β-ol (4), and (22E, 24R)-ergosta-7, 22-dien-3β-ol (5)were isolated. Conclusion Compounds 1 and 2 are new and other compounds are isolated from this fungus of Ganoderma Karst. for the first time.
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[基金项目]
国家自然科学基金面上项目(81172940);中国科学院知识创新项目(KSCX2-YW-G-038,KSCX2-YW-R-194,KZCX2-XB2-15-03);植物化学与西部植物资源持续利用国家重点实验室自主项目基金(P2010-ZZ14)