目的研究水杨酸(SA)对白桦悬浮细胞生长、齐墩果酸积累及防御酶活性的影响。方法在白桦悬浮培养第7、10、13、15天,分别添加不同质量浓度(10、25、50、100、250 mg/L)的SA,进行细胞生长、齐墩果酸量及抗逆酶活性分析。结果添加低质量浓度SA(10～25 mg/L)对细胞活力和生长量影响不大,高质量浓度的SA(100～250 mg/L)处理可显著抑制细胞活力和生长,但可促进齐墩果酸的积累,在第7天添加250 mg/L SA后齐墩果酸量最高,达1.71 mg/g(干质量),是对照的3.1倍;而第7天添加50 mg/L SA后齐墩果酸的产量为0.473 mg,比对照提高了42.04%。悬浮培养第7天添加50 mg/L SA,过氧化氢酶(CAT)和超氧化物歧化酶(SOD)两种酶活性分别在0～24 h和0～48 h比对照显著提高,而PAL活性在SA处理12 h后始终高于对照。结论适宜浓度的SA可刺激白桦细胞防御酶活性提高,并促进白桦悬浮细胞中齐墩果酸的积累。

Objective To study the effects of salicylic acid (SA) on cell growth, oleanolic acid (OA) accumulation, and the activity of defense enzyme in the suspension cells of Betula platyphylla.Methods The cell growth, OA accumulation, and the activity of defense enzyme were studied after adding SA (10, 25, 50, 100, and 250 mg/L) to the suspension culture of B. platyphylla on the day 7, 10, 13, and 15. Results Under low concentration of SA (10―25 mg/L), cell viability and callus growth of B. platyphylla had few change and were significantly inhibited under high concentration of SA (100―250 mg/L), but the accumulation of OA increased significantly and the content of OA reached top at 1.71 mg/g (dry weight) with 250 mg/L SA on day 7 of the cell culture, which was 3.1 times of the control group; When 50 mg/L SA was applied to the cell culture, the production of OA reached 0.473 mg, which was 42.04% higher than that of the conrtol. When it was added on day 7, the activities of CAT and SOD enzymes increased significantly in 0―24 and 0―48 h, respectively. The PAL activity was always higher than the control after 12 h.Conclusion The suitable concentration of SA can increase the activity of defense enzyme and the production of OA in the suspension cells of B. platyphylla.

国家自然科学基金资助项目(31070531);黑龙江省自然科学面上基金项目(C201110);中央高校基本科研业务费专项资金资助(DL12CA04);东北林业大学研究生论文资助项目