[关键词]
[摘要]
目的 多点采样研究并建立党参质量评价体系,采用其对不同来源的党参进行质量差异性分析。方法 对党参的总灰分、酸不溶灰分、醇溶性浸出物进行测定,采用HPLC-DAD对党参炔苷进行测定并建立HPLC指纹图谱;将指标与指纹图谱结合起来建立党参质量评价体系;用建立的质量评价体系对不同来源的党参样品进行质量评价。结果 不同产地灰分和酸不溶灰分检测结果显示,符合《中国药典》2010年版标准;而不同来源党参炔苷量具有显著性差异;不同产地的党参指纹图谱具有9个共有峰,相关系数均在0.8以上,建立的指纹图谱可用于党参质量评价。结论 本研究用多指标检测结合指纹图谱建立的党参质量评价体系可用于不同来源党参的质量评价及质量控制。
[Key word]
[Abstract]
Objective To establish the quality evaluation system for the roots of Codonopsis pilosula by using multipoint sampling and to analyze the quality differences in the roots of C. pilosula from different habitats. Methods To detect the total ash, acid-insoluble ash, and ethanol-soluble extracts; to detect the contents of lobetyolin and establish the HPLC fingerprint for the roots of C. pilosula from different habitats; to combine the indexes of total ash; acid-insoluble ash, ethanol solubility extract, and the content of lobetyolin with HPLC fingerprint to establish the quality evaluation system for the roots of C. pilosula, which was used to assess the quality for the roots of C. pilosula from different habitats. Results The detection results of ash and acid-insoluble ash indicated that these contents in most samples could meet the standard of Chinese Pharmacopeia 2010; There are significant differences among the contents of lobetyolin in different samples; HPLC fingerprint had nine common peaks and the correlation coefficient reached over 0.8; the quality evaluation system could be used to assess the quality for the roots of C. pilosula from different habitats. Conclusion The quality for the roots of C. pilosula from different habitats could be assessed and controlled by the quality evaluation system established by multipoint combined with fingerprints in this research.
[中图分类号]
[基金项目]
国家科技基础性工作专项重点项目(2007FY110800);西北农林科技大学博士科研启动基金(2010BSJJ043)