目的 探讨姜黄素对体外培养大鼠肝星状细胞（HSC）增殖、转化生长因子β1（TGF-β1）及其受体、下游信号通路及效应的影响。方法 培养HSC细胞，给予不同浓度姜黄素处理，采用MTT比色法检测HSC细胞增殖；Hoechst 33342染色检测姜黄素对HSC凋亡的影响；采用免疫细胞化学法检测Smad3、Smad7蛋白表达；用逆转录聚合酶链式反应（RT-PCR）检测TGF-β1及其I、II受体（TβRI、TβRII）和I、III型胶原（Col-I、Col-III）mRNA的表达。结果 姜黄素能抑制HSC细胞增殖，诱导HSC细胞凋亡，免疫组化结果显示姜黄素能抑制HSC表达Smad3，并提高Smad7表达，RT-PCR结果显示姜黄素使HSC细胞TGF-β1及其I、II受体、Col-I、Col-III mRNA表达降低。结论 姜黄素能抑制HSC增殖和活化，抑制TGF-β1及其受体和信号通路，是其抗肝纤维化的作用机制之一。

Objective To observe the inhibition effect of curcumin on hepatic stellate cells (HSC) cell proliferation, mRNA expression of transforming growth factor-beta1 (TGF-β1) and its receptors, and signal transduction. Methods The proliferation of HSC was examined by MTT colorimetric. The apoptosis of HSC was examined by Hoechst 33342; The mRNA levels of TGF-β1, TβRI, TβRII, collagen I (Col-I), and collagen III (Col-III) were examined by RT-PCR; The expression of Smad3 and Smad7 in HSC was examined by immuneohistochemical method. Results Curcumin could inhibit HSC proliferation and induce HSC apoptosis; What’s more, the mRNA levels of TGF-β1, TβRI, TβRII, Col-I, and Col-III, and the expression of Smad3 was significantly decreased by curcumin; The expression of Smad7 was remarkably increased. Conclusion Curcumin could inhibit the proliferation of HSC, inhibit the expression of TGF-β1, and its receptors as well as its signal transduction, which could result in the effect of antihepatic fibrosis.

广东省中医药局项目（2009276）；广东食品药品职业学院院级项目（2007012）