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[摘要]
目的 研究川泽泻质量及其土壤理化性质,分析二者的相关性,为提高川泽泻质量提供依据。方法 采用RP-HPLC测定川泽泻中23-乙酰泽泻醇B的量;参照《中国药典》2010年版的测定方法测定川泽泻水分、醇溶性浸出物、总灰分和酸不溶性灰分量;采用常规方法测定泽泻种植区土壤理化性质。结果 除醇溶性浸出物外,川泽泻含水量、23-乙酰泽泻醇B、总灰分和酸不溶性灰分均符合《中国药典》2010年版的规定;总灰分与醇溶性浸出物显著负相关,相关系数为0.407,与酸不溶性灰分显著正相关,相关系数为0.466;各产地泽泻速效钾量缺乏严重;土壤有机质与碱解氮,全磷与速效磷存在极显著正相关,相关系数分别达到0.555、0.806,全钾与速效钾显著正相关,相关系数为0.433;土壤各理化指标内某一因素对泽泻相应指标的影响受其他土壤因子的影响较大;土壤全钾量对川泽泻各成分量的影响最大,其次为全氮量。结论 各地之间川泽泻质量较优且稳定,可作为制定川泽泻质量标准的依据;川泽泻种植时应选择土壤全钾量、速效钾量较高的地块,以保证川泽泻质量。
[Key word]
[Abstract]
Objective To provide a basis for improving the quality of Alisma orientale in Sichuan Province by studying its quality andrhizosphere soil physicochemical property and analyzing their relevance correlation. Methods Determination of alisol B-23-acetateby RP-HPLC and water, ethanol extracts, total ash, and acid insoluble ash in A. orientale in Sichuan Province was carried outaccording to Chinese Pharmacopoeia 2010. And to investigate the physicochemical property of rhizosphere soil in growing area of A.orientale by the conventional methods. At last, to analyze the data by SPSS 17.0. Results All the contents of water, alisolB-23-acetate, total ash, and acid insoluble ash in A. orientale in Sichuan Province could meet the standard of Chinese Pharmacopoeia2010, except the ethanol extracts. The total ash was significantly negative correlation to ethanol extracts, while significantly positivecorrelation to acid insoluble ash, the relative coefficients were -0.407 and 0.466, respectively. The contents of available K were shortin all soil of growing areas for planting A. orientale. There was extremely significantly positive correlation between soil organic matterand alkali-hydrolysable N, total P, and available P, while significantly positive correlation between total K and available K, the relativecoefficients were 0.555, 0.806, and 0.433, respectively. Every single factor of all soil physicochemical property was affected by othersoil factors highly. The total K of soil had the highest influence on the content of each component in A. orientale in Sichuan Provinceand the following was total N. Conclusion The quality of A. orientale in Sichuan Province in every place is good and steady, whichcould provide the reference for making the quality standard for A. orientale in Sichuan Province. The total K is influenced by theavailable K, but has the significant influence on A. orientale in Sichuan Province. However, all of the fields where planted A. orientalein Sichuan Province are short of available K. Therefore, to ensure the quality of A. orientale in Sichuan Province, we should choose thefields where there are enough total K and available K.
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[基金项目]
公益性行业(农业)科研专项“西南丘陵地区多熟高产高效生态农作制技术体系研究与示范”(200803028)