目的 建立淫羊藿植物PCR-RFLP标记系统关系图。方法 对主要来自于四川、贵州的17种淫羊藿植物进行了系统的PCR-RFLP标记。结果 在8个引物的PCR-RFLP标记分析中,有7个标记能得到1条清晰的谱带;利用12种限制性内切酶对7个标记的扩增产物消化后,在84种引物/酶组合中,共检测到129条酶切片段,其中44条具有多态性;遗传相似系数为0.550～0.988,平均为0.821。结论 该属植物遗传关系与其地理分布关系密切。

Objective To establish the phylogenic map of plants in Epimedium L. based on PCR-RFLP data. Methods Seventeenspecies from Epimedium L. native to Sichuan and Guizhou provinces etc. were studied by means of PCR-RFLP Marker. ResultsAmong the eight PCR-RFLP Markers, seven (87.5%) could produce one distinct band with no polymorphism detected by directelectrophoresis in 2% agrose gels. After the amplified products were digested by 12 restriction enzymes, a total of 129 bands weredetected in 84 PCR-RFLP primer/enzyme combinations. And 44 polymorphic bands accounting for 34.1% have polymorphism. Thegenetic similarity coefficient (Gs) among 17 species from Epimedium L. showed the range from 0.550 to 0.988, with the mean of 0.821.Conclusion It is clear that there are some relationships between the genetic property and its geographic distribution.

贵州省中药现代化专项(黔科合农字[2005]5052号)