目的 制备淫羊藿苷元自微乳,考察其理化性质及体外肠吸收特性。方法 在考察淫羊藿苷元理化性质的基础上,以油酸乙酯为油相,聚山梨酯80为乳化剂,丙三醇为助乳化剂制备淫羊藿苷元自微乳,采用透射电镜及激光粒度分析仪测定其稀释后所得微乳的形态、粒径分布和Zeta电位,并采用CaCO₂细胞模型初步分析其肠吸收特性。结果 所制备的淫羊藿苷元自微乳稀释后,微乳平均粒径为5.6 nm,Zeta电位为30.8 mV,在CaCO₂细胞模型上的表观渗透系数(Papp)为(3.52±0.3)×10 6cm/s。结论 淫羊藿苷元自微乳制剂稳定,体外研究显示自微乳系统能够促进淫羊藿苷元在肠道的吸收。

Objective To prepare icaritin self-microemulsion and investigate its properties and oral absorption characteristics inCaCO₂ monolayer model. Methods To prepare icaritin self-microemulsion with ethyl oleate as oil phase, Polysorbate-80 assurfactant, and glycerol as cosurfactant. The appearance, particle diameter distribution, and Zeta potential of the diluted icaritinself-microemulsion were investigated by transmission electron microscopy and laser grain size analyzer, and the intestinal absorptionwas studied by using CaCO₂ monolayer model. Results The mean particle diameter distribution of the diluted icaritinself-microemulsion was 55.6 nm, with Zeta potential of -30.8 mV. The apparent permeability coefficient (P app ) of the dilutedmicroemulsion in CaCO₂ monolayer model was (3.52 ± 0.3) × 10 ^-6 cm/s. Conclusion The icaritin self-microemulsion is stable andcould significantly improve the absorption of icaritin in the intestinal tract.

国家自然科学基金资助项目(81173557);江苏省中医药领军人才项目(LJ200913);江苏省中医药局资助项目(LZ09063)