目的 制备淫羊藿苷元自微乳，考察其理化性质及体外肠吸收特性。方法 在考察淫羊藿苷元理化性质的基础上，以油酸乙酯为油相，聚山梨酯80为乳化剂，丙三醇为助乳化剂制备淫羊藿苷元自微乳，采用透射电镜及激光粒度分析仪测定其稀释后所得微乳的形态、粒径分布和Zeta电位，并采用Caco-2细胞模型初步分析其肠吸收特性。结果 所制备的淫羊藿苷元自微乳稀释后，微乳平均粒径为55.6 nm，Zeta电位为?30.8 mV，在Caco-2细胞模型上的表观渗透系数（P_app）为（3.52±0.3）×10^?6 cm/s。结论 淫羊藿苷元自微乳制剂稳定，体外研究显示自微乳系统能够促进淫羊藿苷元在肠道的吸收。

Objective To prepare icaritin self-microemulsion and to investigate its properties and oral absorption characteristics in Caco-2 monolayer model. Methods To prepare icaritin self-microemulsion with ethyl oleate as oil phase, Polysorbate-80 as surfactant, and glycerol as cosurfactant. The appearance, particle diameter distribution, and Zeta potential of the diluted icaritin self-microemulsion were investigated by transmission electron microscopy and laser grain size analyzer, and the intestinal absorption was studied by using Caco-2 monolayer model. Results The mean particle diameter distribution of the diluted icaritin self-microemulsion was 55.6 nm，with Zeta potential of ?30.8 mV. The apparent permeability coefficient (P_app) of the diluted microemulsion in Caco-2 monolayer model was (3.52 ± 0.3) × 10^?6 cm/s. Conclusion The icaritin self-microemulsion is stable and could significantly improve the absorption of icaritin in the intestinal tract.

国家自然科学基金资助项目（81173557）；江苏省中医药领军人才项目（LJ200913）；江苏省中医药局资助项目（LZ09063）