目的 通过考察川乌、制川乌、草乌、制草乌、生附子、黑顺片分别与浙贝母、川贝母配伍前后双酯型生物碱的量，分析共煎液中乌头类生物碱之间的转化。方法 采用HPLC及电喷雾质谱技术，对川乌、草乌、生附子及其炮制品与浙贝母、川贝母配伍前后双酯型生物碱进行定量测定。结果 乌头属中药及其炮制品在与浙贝母配伍时能够抑制双酯型生物碱的水解转化，而与川贝母配伍对双酯型生物碱的水解转化则起到促进作用。结论 从化学成分量的变化方面分析，中药“十八反”之“乌头反贝母”并不是绝对的，乌头属中药及其炮制品与浙贝母配伍“相反”，与川贝母配伍“不反”。

Objective To investigate diester-diterpenoid alkaloids content in the decoctions before and after Aconiti Radix, Aconiti Radix Cocta, Aconiti Kusnezoffii Radix, Aconiti Kusnezoffii Radix Cocta, Aconiti Lateralis Radix Praeparata, Radix Aconiti Lateralis Preparata combined with Fritillariae Thunberglii Bulbus and Fritillariae Cirrhosae Bulbus and study the conversion of alkaloids in decoctions. Methods To quantitatively detect the content of diester-diterpenoid alkaloids in decoctions of plants in Aconitum L. and their processed products combined with Fritillariae Thunberglii Bulbus and Fritillariae Cirrhosae Bulbus by HPLC and ESI-MS. Results Decoctions containing Fritillariae Thunberglii Bulbus could inhibit the hydrolysis and conversion of alkaloids, while those containing Fritillariae Cirrhosae Bulbus could promote the transformation of alkaloids and reduce their content. Conclusion Analyses of content changes of three diester-diterpenoid alkaloids indicate that the statement of “aconite counteracts fritillaria” is not comprehensive medicinal plants in Aconitum L. and their processed products combined with Fritillariae Thunberglii Bulbus are counteraction, but with Fritillariae Cirrhosae Bulbus are not.

国家自然科学基金资助项目（30772721）；“973”计划中医理论专项（2006CB504706）