[关键词]
[摘要]
目的 研究不同理化因素对天山雪莲组培苗生长和总黄酮生物合成的影响。方法 测定不同理化因素影响下天山雪莲组培苗干质量和总黄酮量。结果 利于天山雪莲生长及黄酮积累的培养条件:MS培养基附加6-BA 0.5 mg/L,NAA 0.05mg/L,氮源浓度40 mmol/L,NH4+-NO3?比例为15︰25,碳源蔗糖质量浓度40 g/L,pH值5.0~6.0,光照强度2 500 lx,温度25℃。结论 各项最佳培养条件的组合总体上可以促进天山雪莲生长和总黄酮生物合成,氮源、碳源和温度对天山雪莲生长及总黄酮量的影响最为明显。
[Key word]
[Abstract]
Objective To investigate the effects of physicochemical factors on the growth and total flavonoids accumulation of Saussurea involucrate. Methods The dry weight and total flavonoids accumulation in multiple seedings of S. involucrata were measured. Results The optimal cultural condition for the growth and total flavonoid accumulation of S. involucrata was as following: MS culture medium adding 6-BA 0.5 mg/L + NAA 0.05 mg/L, 40 mmol/L of nitrogen source, NH4+-NO3? (15︰25), 40 g/L of carbon sucrose, pH values 5.0-6.0, illumination intensity of 2 500 lx, temperature 25 ℃. Conclusion The optimal culture conditions could significantly improve the growth and total flavonoids accumulation of S. involucrata. And the nitrogen, carbon, and temperature have the most obvious effects on the growth and total flavonoids accumulation of S. involucrata.
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[基金项目]
国际科技合作项目(2009DFA32250)