[关键词]
[摘要]
目的 研究龙葵碱对乳腺癌MCF-7细胞微管系统的影响。方法 MTT法检测龙葵碱对人乳腺癌MCF-7细胞增殖的抑制作用,流式细胞仪分析龙葵碱对MCF-7细胞周期的影响以及细胞内α-微管蛋白及微管相关蛋白(MAP-2)的变化。结果 龙葵碱对MCF-7细胞的IC50为22.08μg/mL,能够将MCF-7细胞阻滞于S期;能够增加MCF-7细胞内α-微管蛋白和MAP-2的量。结论 龙葵碱通过升高MCF-7细胞内的微管蛋白及MAP-2的表达,将MCF-7细胞阻滞于S期,从而抑制乳腺癌MCF-7细胞的生长。
[Key word]
[Abstract]
Objective To investigate the effects of solanine on microtubular system in MCF-7 cell line. Methods Proliferation inhibition of MCF-7 cell line was evaluated by MTT assay. Cell cycle of MCF-7 cells was analyzed and the changes of α-tubulin protein and microtubule-associated protein 2 (MAP-2) protein were detected by flow cytometry. Results The IC50 of MCF-7 cells was 22.08 μg/mL. Solanine could induce MCF-7 cells arrested in S phaseand increase the levels of α-tubulin and MAP-2 in MCF-7 cell line. Conclusion Solanine could inhibit the MCF-7 cell proliferation by increasing α-tubulin and MAP-2 expression and inducing MCF-7 cells arrested in S phase.
[中图分类号]
[基金项目]
国家自然科学基金资助项目(30400591);教育部博士点基金项目(200802400001);黑龙江省博士后资助项目(LBHZ09094);黑龙江省研究生创新基金项目(YJSCX2011-199HSD)