[关键词]
[摘要]
目的 对苦味叶下珠Phyllanthus amarus的化学成分进行研究。方法 采用硅胶柱色谱、Sephadex-20柱色谱、重结晶及制备高效液相色谱等技术提取分离苦味叶下珠的化学成分,采用UV、IR、EI-MS、1H-NMR、13C-NMR、HMQC及HMBC等光谱方法鉴定化合物结构。结果 从苦味叶下珠乙醇提取物的石油醚部位分离得到化合物1~3,分别鉴定为棕榈酸(1)、对羟基苯甲醛(2)、齐墩果酸(3);从二氯甲烷部位分离得到化合物4~9,分别鉴定为stigmast-5-en-3-ol, oleate(4)、4-羟基-3-甲氧基苯甲酸(5)、胡萝卜苷(6)、芹菜素(7)、木犀草素(8)、叶下珠新苷(9)。结论 化合物9是新化合物,命名为叶下珠新苷,化合物4~8为首次从苦味叶下珠中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents of Phyllanthus amarus. Methods Chromatography on silica gel column, Sephadex LH-20 columm, recrystallization, and preparative HPLC technique were used to isolate and purify the compounds. Spectroscopic methods including UV, IR, EI-MS, 1H-NMR, 13C-NMR, HMQC, and HMBC were used to elucidate the structures of compounds. Results Nine compounds were obtained and identified as palmitic acid (1), 4-hydrobenzal dehyde (2), oleanolic acid (3), stigmast-5-en-3-ol, oleate (4), 4-hydroxy-3-methoxy-benzoic acid (5), daucosterol (6), apigenin (7), luteolin (8), and phyllanoside (9). Conclusion Compound 9 is a new compound, named phyllanoside. Compounds 4—8 are isolated from the plant for the first time.
[中图分类号]
[基金项目]